A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules

R J Goodwin, L Macintyre, D G Watson, S P Scullion, A R Pitt

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Matrix application continues to be a critical step in sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). Imaging of small molecules such as drugs and metabolites is particularly problematic because the commonly used washing steps to remove salts are usually omitted as they may also remove the analyte, and analyte spreading is more likely with conventional wet matrix application methods. We have developed a method which uses the application of matrix as a dry, finely divided powder, here referred to as dry matrix application, for the imaging of drug compounds. This appears to offer a complementary method to wet matrix application for the MALDI-MSI of small molecules, with the alternative matrix application techniques producing different ion profiles, and allows the visualization of compounds not observed using wet matrix application methods. We demonstrate its value in imaging clozapine from rat kidney and 4-bromophenyl-1,4-diazabicyclo(3.2.2)nonane-4-carboxylic acid from rat brain. In addition, exposure of the dry matrix coated sample to a saturated moist atmosphere appears to enhance the visualization of a different set of molecules.
Original languageEnglish
Pages (from-to)1682-1686
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume24
Issue number11
DOIs
Publication statusPublished - Jun 2010

Keywords

  • animals
  • bicyclo compounds, heterocyclic
  • brain chemistry
  • clozapine
  • kidney
  • male
  • rats
  • rats, inbred BB
  • rats, wistar
  • spectrometry, mass, matrix-assisted laser desorption-ionization

Fingerprint Dive into the research topics of 'A solvent-free matrix application method for matrix-assisted laser desorption/ionization imaging of small molecules'. Together they form a unique fingerprint.

  • Cite this