A single luminally continuous sarcoplasmic reticulum with apparently separate Ca2+ stores in smooth muscle

J.G. McCarron, Marnie Olson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Whether or not the sarcoplasmic reticulum (SR) is a continuous, interconnected network surrounding a single lumen or comprises multiple, separate Ca2+ pools was investigated in voltage-clamped single smooth muscle cells using local photolysis of caged compounds and Ca2+ imaging. The entire SR could be depleted or refilled from one small site via either inositol 1,4,5-trisphosphate receptors (IP3R) or ryanodine receptors (RyR) suggesting the SR is luminally continuous and that Ca2+ may diffuse freely throughout. Notwithstanding, regulation of the opening of RyR and IP3R, by the [Ca2+] within the SR, may create several apparent SR elements with various receptor arrangements. IP3R and RyR may appear to exist entirely on a single store, and there may seem to be additional SR elements that express either only RyR or only IP3R. The various SR receptor arrangements and apparently separate Ca2+ storage elements exist in a single luminally continuous SR entity.
LanguageEnglish
Pages7206-7218
Number of pages13
JournalJournal of Biological Chemistry
Volume283
Issue number11
DOIs
Publication statusPublished - 14 Mar 2008

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Ryanodine Receptor Calcium Release Channel
Sarcoplasmic Reticulum
Smooth Muscle
Muscle
Inositol 1,4,5-Trisphosphate Receptors
Photolysis
Cells
Imaging techniques
Electric potential
Smooth Muscle Myocytes

Keywords

  • sarcoplasmic reticulum
  • smooth muscle cells
  • Ca2+ stores
  • photolysis
  • caged compounds

Cite this

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A single luminally continuous sarcoplasmic reticulum with apparently separate Ca2+ stores in smooth muscle. / McCarron, J.G.; Olson, Marnie.

In: Journal of Biological Chemistry, Vol. 283, No. 11, 14.03.2008, p. 7206-7218.

Research output: Contribution to journalArticle

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T1 - A single luminally continuous sarcoplasmic reticulum with apparently separate Ca2+ stores in smooth muscle

AU - McCarron, J.G.

AU - Olson, Marnie

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AB - Whether or not the sarcoplasmic reticulum (SR) is a continuous, interconnected network surrounding a single lumen or comprises multiple, separate Ca2+ pools was investigated in voltage-clamped single smooth muscle cells using local photolysis of caged compounds and Ca2+ imaging. The entire SR could be depleted or refilled from one small site via either inositol 1,4,5-trisphosphate receptors (IP3R) or ryanodine receptors (RyR) suggesting the SR is luminally continuous and that Ca2+ may diffuse freely throughout. Notwithstanding, regulation of the opening of RyR and IP3R, by the [Ca2+] within the SR, may create several apparent SR elements with various receptor arrangements. IP3R and RyR may appear to exist entirely on a single store, and there may seem to be additional SR elements that express either only RyR or only IP3R. The various SR receptor arrangements and apparently separate Ca2+ storage elements exist in a single luminally continuous SR entity.

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