Projects per year
Abstract
Multi-wavelength standing wave (SW) microscopy and interference reflection microscopy (IRM) are powerful techniques that use optical interference to study topographical structure. However, the use of more than two wavelengths to image the complex cell surface results in complicated topographical maps and it can be difficult to resolve the three-dimensional contours. We present a simple image processing method to reduce the thickness and spacing of antinodal fringes in multiwavelength interference microscopy by up to a factor of two to produce clearer and more precise topographical maps of cellular structures. We first demonstrate this improvement using model non-biological specimens,
and we subsequently demonstrate the benefit of our method for reducing the ambiguity of surface topography and revealing obscured features in live and
fixed cell specimens.
and we subsequently demonstrate the benefit of our method for reducing the ambiguity of surface topography and revealing obscured features in live and
fixed cell specimens.
Original language | English |
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Pages (from-to) | 1092-1095 |
Number of pages | 4 |
Journal | Optics Letters |
Volume | 48 |
Issue number | 5 |
DOIs | |
Publication status | Published - 16 Feb 2023 |
Keywords
- interference reflection microscopy (IRM)
- cellular structures
- image processing
- TartanSW microscopy
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Dive into the research topics of 'A simple image processing pipeline to sharpen topology maps in multi-wavelength interference microscopy'. Together they form a unique fingerprint.Projects
- 4 Finished
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FASPRI:a new method for increased spatial resolution in surface plasmon imaging of unlabelled living cells.
McConnell, G. (Principal Investigator)
BBSRC (Biotech & Biological Sciences Research Council)
1/01/21 → 31/03/22
Project: Research
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TartanSW: a new method for spectrally-resolved standing wave cell microscopy and mesoscopy
McConnell, G. (Principal Investigator) & Bushell, T. (Co-investigator)
BBSRC (Biotech & Biological Sciences Research Council)
1/08/17 → 6/02/20
Project: Research
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EPSRC Centre for Doctoral Training in Optical Medical Imaging - Training the next generation of scientific entrepreneurs in healthcare technologies (OPTIMA)
Graham, D. (Principal Investigator) & Faulds, K. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/05/14 → 31/10/23
Project: Research - Studentship