A simple hand-held magnet array for efficient and reproducible SABRE hyperpolarisation using manual sample shaking

Peter M. Richardson, Scott Jackson, Andrew Parrott, Alison Nordon, Simon B. Duckett, Meghan E. Halse

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Signal amplification by reversible exchange (SABRE) is a hyperpolarisation technique that catalytically transfers nuclear polarisation from parahydrogen, the singlet nuclear isomer of H2, to a substrate in solution. The SABRE exchange reaction is carried out in a polarisation transfer field (PTF) of tens of gauss before transfer to a stronger magnetic field for NMR detection. In the simplest implementation, polarisation transfer is achieved by shaking the sample in the stray field of a superconducting NMR magnet. While convenient, this method suffers from limited reproducibility and cannot be used with NMR spectrometers that do not have appreciable stray fields, such as benchtop instruments. Here we use a simple hand-held permanent magnet array to provide the necessary PTF during sample shaking. We find that the use of this array provides a 25% increase in SABRE enhancement over the stray field approach, while also providing improved reproducibility. Arrays with a range of PTFs were tested and the PTF-dependent SABRE enhancements were found to be in excellent agreement with comparable experiments carried out using an automated flow system where an electromagnet is used to generate the PTF. We anticipate that this approach will improve the efficiency and reproducibility of SABRE experiments carried out using manual shaking and will be particularly useful for benchtop NMR, where a suitable stray field is not readily accessible. The ability to construct arrays with a range of PTFs will also enable the rapid optimisation of SABRE enhancement as function of PTF for new substrate and catalyst systems.
LanguageEnglish
Pages641-650
Number of pages10
JournalMagnetic Resonance in Chemistry
Volume56
Issue number7
Early online date28 Nov 2017
DOIs
Publication statusPublished - 1 Jul 2018

Fingerprint

Magnets
Amplification
Polarization
Nuclear magnetic resonance
Electromagnets
Substrates
Isomers
Permanent magnets
Spectrometers
Ion exchange
Experiments
Magnetic fields
Catalysts

Keywords

  • NMR
  • 1H
  • hyperpolarisation
  • parahydrogen
  • signal amplification by reversible exchange (SABRE)
  • benchtop NMR
  • Halbach array
  • polarisation transfer field

Cite this

Richardson, Peter M. ; Jackson, Scott ; Parrott, Andrew ; Nordon, Alison ; Duckett, Simon B. ; Halse, Meghan E. / A simple hand-held magnet array for efficient and reproducible SABRE hyperpolarisation using manual sample shaking. In: Magnetic Resonance in Chemistry . 2018 ; Vol. 56, No. 7. pp. 641-650.
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A simple hand-held magnet array for efficient and reproducible SABRE hyperpolarisation using manual sample shaking. / Richardson, Peter M.; Jackson, Scott; Parrott, Andrew; Nordon, Alison; Duckett, Simon B.; Halse, Meghan E.

In: Magnetic Resonance in Chemistry , Vol. 56, No. 7, 01.07.2018, p. 641-650.

Research output: Contribution to journalArticle

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T1 - A simple hand-held magnet array for efficient and reproducible SABRE hyperpolarisation using manual sample shaking

AU - Richardson, Peter M.

AU - Jackson, Scott

AU - Parrott, Andrew

AU - Nordon, Alison

AU - Duckett, Simon B.

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AB - Signal amplification by reversible exchange (SABRE) is a hyperpolarisation technique that catalytically transfers nuclear polarisation from parahydrogen, the singlet nuclear isomer of H2, to a substrate in solution. The SABRE exchange reaction is carried out in a polarisation transfer field (PTF) of tens of gauss before transfer to a stronger magnetic field for NMR detection. In the simplest implementation, polarisation transfer is achieved by shaking the sample in the stray field of a superconducting NMR magnet. While convenient, this method suffers from limited reproducibility and cannot be used with NMR spectrometers that do not have appreciable stray fields, such as benchtop instruments. Here we use a simple hand-held permanent magnet array to provide the necessary PTF during sample shaking. We find that the use of this array provides a 25% increase in SABRE enhancement over the stray field approach, while also providing improved reproducibility. Arrays with a range of PTFs were tested and the PTF-dependent SABRE enhancements were found to be in excellent agreement with comparable experiments carried out using an automated flow system where an electromagnet is used to generate the PTF. We anticipate that this approach will improve the efficiency and reproducibility of SABRE experiments carried out using manual shaking and will be particularly useful for benchtop NMR, where a suitable stray field is not readily accessible. The ability to construct arrays with a range of PTFs will also enable the rapid optimisation of SABRE enhancement as function of PTF for new substrate and catalyst systems.

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KW - parahydrogen

KW - signal amplification by reversible exchange (SABRE)

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