A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons

K. Chung, J. Lee, J. E. Lee, J. Y. Lee, S. Moon, K. H. A. Lau, D. Kim, D. H. Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the coupling-induced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).
LanguageEnglish
Pages1074-1080
Number of pages7
JournalSensors and Actuators B: Chemical
Volume176
Early online date12 Oct 2012
DOIs
Publication statusPublished - 2013

Fingerprint

Plasmons
plasmons
DNA
deoxyribonucleic acid
Nanoparticles
nanoparticles
augmentation
sensitivity
Streptavidin
data simulation
Surface plasmon resonance
Biomolecules
surface plasmon resonance
affinity
Assays
Transmission electron microscopy
reflectance
transmission electron microscopy
shift
Computer simulation

Keywords

  • SPR sensor
  • plasmonic coupling
  • DNA hybridization
  • Au nanoparticles
  • Au-amplified
  • SPR fluorescence
  • spectroscopy
  • biosensor

Cite this

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title = "A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons",
abstract = "This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the coupling-induced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).",
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A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons. / Chung, K.; Lee, J.; Lee, J. E.; Lee, J. Y.; Moon, S.; Lau, K. H. A.; Kim, D.; Kim, D. H.

In: Sensors and Actuators B: Chemical, Vol. 176, 2013, p. 1074-1080.

Research output: Contribution to journalArticle

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AU - Chung, K.

AU - Lee, J.

AU - Lee, J. E.

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AU - Moon, S.

AU - Lau, K. H. A.

AU - Kim, D.

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