The identification of low-molecular-weight protein inhibitors for functional studies as well as for the generation of lead drug compounds is one of the most important tasks in chemical biology. We describe a generalized assay that utilizes inhibitory aptamers for the identification of small molecules with aptamer-inherited properties. These compounds share the functional profile of the aptamers and thus are considered as potent protein inhibitors.
- high-throughput screening
- protein inhibitors
Mayer, G., Faulhammer, D., Grättinger, M., Fessele, S., & Blind, M. (2009). A RNA-based approach towards small molecule inhibitors. Chembiochem, 10(12), 1993-1996. https://doi.org/10.1002/cbic.200900325