A recovery model of minimally invasive cardiopulmonary bypass in the rat

T. Modine, R. Azzaoui, G. Fayad, D. Lacroix, R. Bordet, H. Warembourg, T. Gourlay

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    This study was undertaken to develop a rodent (rat) model of cardiopulmonary bypass (CPB) that has been designed to mimic functionally the minimally invasive clinical setting. The circuit is similar to the clinical model in terms of its construction, configuration, material surface area to blood volume ratio, and priming volume to blood ratio. The overall priming volume was 10 mL. Thirty-six male Sprague-Dawley rats (422±32 g) were anaesthetized while maintaining spontaneous ventilation. Anticoagulation was achieved with heparin (500 IU/kg). Blood arterial pressure was monitored continuously. Normal central temperature was maintained throughout. Intermittent arterial blood gas levels also were monitored. All animals were cannulated in preparation for CPB; however, CPB, utilizing a double roller pump and a flow rate of 100 mL/kg/minute for 60 min, was initiated in only 18 animals, the remaining 18 animals acting as non-CPB controls (Sham). The animals were haemodynamically stable. After the operative procedure, the animals were allowed to recover from the anaesthesia and, after transfer to a recovery facility, were monitored for a period of 1 week. There were no differences between the groups in terms of blood gas analysis and blood pressure data; all animals survived the procedure and had an uneventful follow-up. Differences were found between the CPB animals and the Sham group in terms of TNF{alpha}, used as a marker of inflammatory processes. This trend tends to support this model as an analogue for the clinical scenario for future studies of CPB-related inflammation. Overall, the CPB procedure was easy to perform and was associated with excellent survival. This recovery model is an effective tool to perform pathophysiological studies associated with minimally invasive CPB.
    LanguageEnglish
    Pages87-92
    Number of pages5
    JournalPerfusion
    Volume21
    Issue number2
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Cardiopulmonary Bypass
    Rats
    Animals
    animal
    Recovery
    Blood
    Blood pressure
    Blood Volume
    Blood gas analysis
    Blood Gas Analysis
    Operative Surgical Procedures
    Ventilation
    Sprague Dawley Rats
    Heparin
    Rodentia
    Arterial Pressure
    Anesthesia
    Group
    Tumor Necrosis Factor-alpha
    Gases

    Keywords

    • cardiopulmonary bypass
    • perfusion
    • rat
    • heart disease

    Cite this

    Modine, T., Azzaoui, R., Fayad, G., Lacroix, D., Bordet, R., Warembourg, H., & Gourlay, T. (2006). A recovery model of minimally invasive cardiopulmonary bypass in the rat. Perfusion, 21(2), 87-92. https://doi.org/10.1191/0267659106pf854oa
    Modine, T. ; Azzaoui, R. ; Fayad, G. ; Lacroix, D. ; Bordet, R. ; Warembourg, H. ; Gourlay, T. / A recovery model of minimally invasive cardiopulmonary bypass in the rat. In: Perfusion. 2006 ; Vol. 21, No. 2. pp. 87-92.
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    abstract = "This study was undertaken to develop a rodent (rat) model of cardiopulmonary bypass (CPB) that has been designed to mimic functionally the minimally invasive clinical setting. The circuit is similar to the clinical model in terms of its construction, configuration, material surface area to blood volume ratio, and priming volume to blood ratio. The overall priming volume was 10 mL. Thirty-six male Sprague-Dawley rats (422±32 g) were anaesthetized while maintaining spontaneous ventilation. Anticoagulation was achieved with heparin (500 IU/kg). Blood arterial pressure was monitored continuously. Normal central temperature was maintained throughout. Intermittent arterial blood gas levels also were monitored. All animals were cannulated in preparation for CPB; however, CPB, utilizing a double roller pump and a flow rate of 100 mL/kg/minute for 60 min, was initiated in only 18 animals, the remaining 18 animals acting as non-CPB controls (Sham). The animals were haemodynamically stable. After the operative procedure, the animals were allowed to recover from the anaesthesia and, after transfer to a recovery facility, were monitored for a period of 1 week. There were no differences between the groups in terms of blood gas analysis and blood pressure data; all animals survived the procedure and had an uneventful follow-up. Differences were found between the CPB animals and the Sham group in terms of TNF{alpha}, used as a marker of inflammatory processes. This trend tends to support this model as an analogue for the clinical scenario for future studies of CPB-related inflammation. Overall, the CPB procedure was easy to perform and was associated with excellent survival. This recovery model is an effective tool to perform pathophysiological studies associated with minimally invasive CPB.",
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    Modine, T, Azzaoui, R, Fayad, G, Lacroix, D, Bordet, R, Warembourg, H & Gourlay, T 2006, 'A recovery model of minimally invasive cardiopulmonary bypass in the rat' Perfusion, vol. 21, no. 2, pp. 87-92. https://doi.org/10.1191/0267659106pf854oa

    A recovery model of minimally invasive cardiopulmonary bypass in the rat. / Modine, T.; Azzaoui, R.; Fayad, G.; Lacroix, D.; Bordet, R.; Warembourg, H.; Gourlay, T.

    In: Perfusion, Vol. 21, No. 2, 2006, p. 87-92.

    Research output: Contribution to journalArticle

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    AU - Modine, T.

    AU - Azzaoui, R.

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    KW - cardiopulmonary bypass

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    Modine T, Azzaoui R, Fayad G, Lacroix D, Bordet R, Warembourg H et al. A recovery model of minimally invasive cardiopulmonary bypass in the rat. Perfusion. 2006;21(2):87-92. https://doi.org/10.1191/0267659106pf854oa