A rapid and sensitive method for the determination of the amount of theophylline in blood spots

D.G. Watson, E.D.J. Oliveira, A.C. Boyter, K.D. Dagg

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine the amount of theophylline in blood spots. The lowest level of theophylline analysed in a blood spot was 15 ng extracted into 250 μL and this was still considerably above the limit of quantification (3 ng in 250 μL). The levels of theophylline in blood spots correlated well with theophylline levels in plasma samples obtained from the same patients. The assay might be of use in therapeutic drug monitoring of theophylline and blood spot sampling could be applied to other drugs where therapeutic monitoring is required.
LanguageEnglish
Pages413-416
Number of pages3
JournalJournal of Pharmacy and Pharmacology
Volume53
Issue number3
DOIs
Publication statusPublished - Mar 2001

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Theophylline
Drug Monitoring
Liquid Chromatography
Mass Spectrometry

Keywords

  • theophylline
  • blood spots
  • therapeutic monitoring

Cite this

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abstract = "Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine the amount of theophylline in blood spots. The lowest level of theophylline analysed in a blood spot was 15 ng extracted into 250 μL and this was still considerably above the limit of quantification (3 ng in 250 μL). The levels of theophylline in blood spots correlated well with theophylline levels in plasma samples obtained from the same patients. The assay might be of use in therapeutic drug monitoring of theophylline and blood spot sampling could be applied to other drugs where therapeutic monitoring is required.",
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A rapid and sensitive method for the determination of the amount of theophylline in blood spots. / Watson, D.G.; Oliveira, E.D.J.; Boyter, A.C.; Dagg, K.D.

In: Journal of Pharmacy and Pharmacology, Vol. 53, No. 3, 03.2001, p. 413-416.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A rapid and sensitive method for the determination of the amount of theophylline in blood spots

AU - Watson, D.G.

AU - Oliveira, E.D.J.

AU - Boyter, A.C.

AU - Dagg, K.D.

PY - 2001/3

Y1 - 2001/3

N2 - Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine the amount of theophylline in blood spots. The lowest level of theophylline analysed in a blood spot was 15 ng extracted into 250 μL and this was still considerably above the limit of quantification (3 ng in 250 μL). The levels of theophylline in blood spots correlated well with theophylline levels in plasma samples obtained from the same patients. The assay might be of use in therapeutic drug monitoring of theophylline and blood spot sampling could be applied to other drugs where therapeutic monitoring is required.

AB - Monitoring of drugs (such as theophylline) with a narrow therapeutic window could be simplified if patients were able to submit blood spots for analysis. This could reduce clinic attendance for venous blood sampling and save staff time. A rapid sensitive method utilizing liquid chromatography-mass spectrometry has been developed to determine the amount of theophylline in blood spots. The lowest level of theophylline analysed in a blood spot was 15 ng extracted into 250 μL and this was still considerably above the limit of quantification (3 ng in 250 μL). The levels of theophylline in blood spots correlated well with theophylline levels in plasma samples obtained from the same patients. The assay might be of use in therapeutic drug monitoring of theophylline and blood spot sampling could be applied to other drugs where therapeutic monitoring is required.

KW - theophylline

KW - blood spots

KW - therapeutic monitoring

U2 - 10.1211/0022357011775497

DO - 10.1211/0022357011775497

M3 - Article

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SP - 413

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JO - Journal of Pharmacy and Pharmacology

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SN - 0022-3573

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