A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy

A. Wright, S.P. Poland, J. Vijverberg, J.M. Girkin, C. Dainty (Editor)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We report on a practical method for the implementation of Adaptive Optics in optical sectioning microscopy to remove system and sample induced aberrations. Correcting for induced aberrations on a pixel-by-pixel basis would take in excess of 4 minutes and greatly increase the risk of sample damage due to photo-bleaching and photo-toxicity; this is clearly an impractical approach. We show that a single aberration correction per optical slice is adequate to significantly improve the image quality across the whole field of view. We present results illustrating the success of this method for a sample scanning and beam scanning system using confocal and multiphoton microscopy.
Original languageEnglish
Title of host publicationAdaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop
Place of PublicationLondon, United Kingdom
Pages382-387
Number of pages5
Publication statusPublished - 2007

Keywords

  • adaptive optics
  • optical sectioning microscopy
  • aberrations
  • photo-bleaching
  • photo-toxicity
  • beam scanning system
  • confocal
  • multiphoton

Cite this

Wright, A., Poland, S. P., Vijverberg, J., Girkin, J. M., & Dainty, C. (Ed.) (2007). A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop (pp. 382-387).