A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy

A. Wright, S.P. Poland, J. Vijverberg, J.M. Girkin, C. Dainty (Editor)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

We report on a practical method for the implementation of Adaptive Optics in optical sectioning microscopy to remove system and sample induced aberrations. Correcting for induced aberrations on a pixel-by-pixel basis would take in excess of 4 minutes and greatly increase the risk of sample damage due to photo-bleaching and photo-toxicity; this is clearly an impractical approach. We show that a single aberration correction per optical slice is adequate to significantly improve the image quality across the whole field of view. We present results illustrating the success of this method for a sample scanning and beam scanning system using confocal and multiphoton microscopy.
LanguageEnglish
Title of host publicationAdaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop
Place of PublicationLondon, United Kingdom
Pages382-387
Number of pages5
Publication statusPublished - 2007

Fingerprint

adaptive optics
aberration
microscopy
pixels
scanning
bleaching
toxicity
field of view
damage

Keywords

  • adaptive optics
  • optical sectioning microscopy
  • aberrations
  • photo-bleaching
  • photo-toxicity
  • beam scanning system
  • confocal
  • multiphoton

Cite this

Wright, A., Poland, S. P., Vijverberg, J., Girkin, J. M., & Dainty, C. (Ed.) (2007). A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop (pp. 382-387). London, United Kingdom.
Wright, A. ; Poland, S.P. ; Vijverberg, J. ; Girkin, J.M. ; Dainty, C. (Editor). / A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. London, United Kingdom, 2007. pp. 382-387
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Wright, A, Poland, SP, Vijverberg, J, Girkin, JM & Dainty, C (ed.) 2007, A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. in Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. London, United Kingdom, pp. 382-387.

A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. / Wright, A.; Poland, S.P.; Vijverberg, J.; Girkin, J.M.; Dainty, C. (Editor).

Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. London, United Kingdom, 2007. p. 382-387.

Research output: Chapter in Book/Report/Conference proceedingChapter

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KW - adaptive optics

KW - optical sectioning microscopy

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KW - photo-toxicity

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Wright A, Poland SP, Vijverberg J, Girkin JM, Dainty C, (ed.). A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. London, United Kingdom. 2007. p. 382-387