A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity

A Aneiros, I García, J R Martínez, A L Harvey, A J Anderson, D L Marshall, A Engström, U Hellman, E Karlsson

Research output: Contribution to journalArticle

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Abstract

A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).
LanguageEnglish
Pages86-92
Number of pages7
JournalBBA - Biochimica et Biophysica Acta
Volume1157
Issue number1
DOIs
Publication statusPublished - 7 May 1993

Fingerprint

Sea Anemones
Potassium Channels
Amino Acid Sequence
Neuromuscular Junction
Spinal Ganglia
Disulfides
Acetylcholine
Neurons
Peptides
Membranes
Brain

Keywords

  • amino acid sequence
  • animals
  • cultured cells
  • chickens
  • gel chromatography
  • cnidaria
  • cnidarian venoms
  • mass spectrometry
  • molecular sequence data
  • neuromuscular junction
  • potassium channels
  • rats
  • amino acid sequence homology

Cite this

Aneiros, A ; García, I ; Martínez, J R ; Harvey, A L ; Anderson, A J ; Marshall, D L ; Engström, A ; Hellman, U ; Karlsson, E. / A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity. In: BBA - Biochimica et Biophysica Acta. 1993 ; Vol. 1157, No. 1. pp. 86-92.
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abstract = "A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).",
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A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity. / Aneiros, A; García, I; Martínez, J R; Harvey, A L; Anderson, A J; Marshall, D L; Engström, A; Hellman, U; Karlsson, E.

In: BBA - Biochimica et Biophysica Acta, Vol. 1157, No. 1, 07.05.1993, p. 86-92.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity

AU - Aneiros, A

AU - García, I

AU - Martínez, J R

AU - Harvey, A L

AU - Anderson, A J

AU - Marshall, D L

AU - Engström, A

AU - Hellman, U

AU - Karlsson, E

PY - 1993/5/7

Y1 - 1993/5/7

N2 - A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).

AB - A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).

KW - amino acid sequence

KW - animals

KW - cultured cells

KW - chickens

KW - gel chromatography

KW - cnidaria

KW - cnidarian venoms

KW - mass spectrometry

KW - molecular sequence data

KW - neuromuscular junction

KW - potassium channels

KW - rats

KW - amino acid sequence homology

U2 - 10.1016/0304-4165(93)90082-J

DO - 10.1016/0304-4165(93)90082-J

M3 - Article

VL - 1157

SP - 86

EP - 92

JO - BBA - Biochimica et Biophysica Acta

T2 - BBA - Biochimica et Biophysica Acta

JF - BBA - Biochimica et Biophysica Acta

SN - 0006-3002

IS - 1

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