Abstract
A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).
Original language | English |
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Pages (from-to) | 86-92 |
Number of pages | 7 |
Journal | BBA - Biochimica et Biophysica Acta |
Volume | 1157 |
Issue number | 1 |
DOIs | |
Publication status | Published - 7 May 1993 |
Keywords
- amino acid sequence
- animals
- cultured cells
- chickens
- gel chromatography
- cnidaria
- cnidarian venoms
- mass spectrometry
- molecular sequence data
- neuromuscular junction
- potassium channels
- rats
- amino acid sequence homology