A potassium channel toxin from the secretion of the sea anemone Bunodosoma granulifera. Isolation, amino acid sequence and biological activity

A Aneiros, I García, J R Martínez, A L Harvey, A J Anderson, D L Marshall, A Engström, U Hellman, E Karlsson

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Abstract

A peptide toxin affecting potassium channels was isolated from the sea anemone Bunodosoma granulifera. It facilitates acetylcholine release at avian neuromuscular junctions, competes with dendrotoxin I, a probe for voltage-dependent potassium channels, for binding to synaptosomal membranes of rat brain with a Ki of 0.7 nM and suppresses K+ currents in rat dorsal root ganglion neurones in culture. It represents a new structural type of potassium channel toxin with the sequence V1RCDWFKETA10CRHAKSLGNC20RTSQKYRANC30AKTLQCC37 (M(r) 4275, three disulfides).
Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalBBA - Biochimica et Biophysica Acta
Volume1157
Issue number1
DOIs
Publication statusPublished - 7 May 1993

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Keywords

  • amino acid sequence
  • animals
  • cultured cells
  • chickens
  • gel chromatography
  • cnidaria
  • cnidarian venoms
  • mass spectrometry
  • molecular sequence data
  • neuromuscular junction
  • potassium channels
  • rats
  • amino acid sequence homology

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