Projects per year
We present a novel 3D PAM system with MEMS optical scanning and fibre tip transducer detection to provide an all-optical system for in vitro cellular and sub-cellular resolution imaging. A 1 mm diameter, electrostatically actuated 2-axis MEMS scanner (Mirrorcle MEMS) provides optical scan angles of ±10°, allowing a large field of view without motion induced artefacts caused by stage scanning. An additional focus tunable lens allows the excitation beam to be scanned in the axial direction. The flexibility of being able to rapidly scan the focus of the excitation beam in 3-dimensions allows selective imaging of regions of interest within the samples. The 20 MHz fibre tip transducers allow for a variety of sample chambers to be used including well plates and petri-dishes, providing increased flexibility in terms of sample mounting and preparation.
The flexibility and small size of the system make it possible to integrate with other imaging modalities such as lightsheet microscopy to harness both fluorescence labelled and label-free contrast of 3D samples.
- photoacoustic microscopy
- fibre tip transducer
- 3D scanning
- Microelectromechanical Systems
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1/10/18 → 1/07/22
Project: Research Studentship - Internally Allocated
1/10/16 → 30/09/21
Project: Research Fellowship