A novel taxonomic marker that discriminates between morphologically complex actinomycetes

Geneviève Girard, Bjørn Traag, Vartul Sangal, Nadine Mascini, Paul Hoskisson, Michael Goodfellow, G. van Wezel

Research output: Contribution to journalArticle

35 Citations (Scopus)
76 Downloads (Pure)

Abstract

In the era where large whole genome bacterial data sets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins (SALPs) are developmental regulatory proteins in sporulating actinomycete, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here we present a novel method to classify actinomycetes, based on the extraordinary way the SsgA and SsgB proteins are conserved. The almost complete conservation of the SsgB amino acid sequence between members of the same genus, and its high divergence even between closely related genera, provides high quality data for the classification of morphologically complex actinomycetes. Our analysis validates Kitasatospora as a sister genus to Streptomyces in the family Streptomycetaceae and suggests that Micromonospora, Salinispora and Verrucosispora may represent different clades of the same genus. It is also apparent that the amino-acid sequence of SsgA is an accurate determinant for the ability of streptomycetes to produce submerged spores, dividing the phylogenetic tree of streptomycetes into LSp (liquid culture sporulation) and NLSp (no liquid culture sporulation) branches. A new phylogenetic tree of industrially relevant actinomycetes is presented and compared to that based on 16S rRNA sequences.
Original languageEnglish
Number of pages14
JournalOpen Biology
Volume3
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Actinobacteria
Streptomycetaceae
16S Ribosomal RNA
Amino Acids
Amino Acid Sequence
Proteins
Micromonospora
Liquids
RNA Sequence Analysis
Bacterial Genomes
Conservation
Streptomyces
Genes
Spores
Cells
Cell Division

Keywords

  • kitasatospora
  • streptomyces
  • streptomycetaceae
  • liquid culture sporulation
  • phylogenetic tree
  • novel taxonomic marker
  • morphologically complex actinomycetes

Cite this

Girard, Geneviève ; Traag, Bjørn ; Sangal, Vartul ; Mascini, Nadine ; Hoskisson, Paul ; Goodfellow, Michael ; van Wezel, G. / A novel taxonomic marker that discriminates between morphologically complex actinomycetes. In: Open Biology. 2013 ; Vol. 3, No. 10.
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Girard, G, Traag, B, Sangal, V, Mascini, N, Hoskisson, P, Goodfellow, M & van Wezel, G 2013, 'A novel taxonomic marker that discriminates between morphologically complex actinomycetes', Open Biology, vol. 3, no. 10. https://doi.org/10.1098/rsob.130073

A novel taxonomic marker that discriminates between morphologically complex actinomycetes. / Girard, Geneviève; Traag, Bjørn ; Sangal, Vartul; Mascini, Nadine; Hoskisson, Paul; Goodfellow, Michael; van Wezel, G.

In: Open Biology, Vol. 3, No. 10, 10.2013.

Research output: Contribution to journalArticle

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T1 - A novel taxonomic marker that discriminates between morphologically complex actinomycetes

AU - Girard, Geneviève

AU - Traag, Bjørn

AU - Sangal, Vartul

AU - Mascini, Nadine

AU - Hoskisson, Paul

AU - Goodfellow, Michael

AU - van Wezel, G.

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AB - In the era where large whole genome bacterial data sets are generated routinely, rapid and accurate molecular systematics is becoming increasingly important. However, 16S ribosomal RNA sequencing does not always offer sufficient resolution to discriminate between closely related genera. The SsgA-like proteins (SALPs) are developmental regulatory proteins in sporulating actinomycete, whereby SsgB actively recruits FtsZ during sporulation-specific cell division. Here we present a novel method to classify actinomycetes, based on the extraordinary way the SsgA and SsgB proteins are conserved. The almost complete conservation of the SsgB amino acid sequence between members of the same genus, and its high divergence even between closely related genera, provides high quality data for the classification of morphologically complex actinomycetes. Our analysis validates Kitasatospora as a sister genus to Streptomyces in the family Streptomycetaceae and suggests that Micromonospora, Salinispora and Verrucosispora may represent different clades of the same genus. It is also apparent that the amino-acid sequence of SsgA is an accurate determinant for the ability of streptomycetes to produce submerged spores, dividing the phylogenetic tree of streptomycetes into LSp (liquid culture sporulation) and NLSp (no liquid culture sporulation) branches. A new phylogenetic tree of industrially relevant actinomycetes is presented and compared to that based on 16S rRNA sequences.

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KW - streptomycetaceae

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