A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans

Anna O. Avrova, Stephen C. Whisson, Leighton Pritchard, Eduard Venter, Sergio De Luca, Ingo Hein, Paul R. J. Birch

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Phytophthora infestans is the cause of late blight, a devastating and re-emerging disease of potato. Significant advances have been made in understanding the biology of P. infestans, and in the development of molecular tools to study this oomycete. Nevertheless, little is known about the molecular bases of the establishment or development of disease in this hemibiotrophic pathogen. Suppression subtractive hybridization (SSH) was used to generate cDNA enriched for sequences upregulated during potato infection. To identify pathogen-derived cDNAs, and eliminate host sequences from further study, SSH cDNA was hybridized to a P. infestans bacterial artificial chromosome library. A new gene family was identified called Pinci1, comprising more than 400 members arranged in clusters of up to nine copies throughout the P. infestans draft genome sequence. Real-time RT-PCR was used to quantify the expression of five classes of transcript within the family, relative to the constitutively expressed PiactA gene, and it revealed them to be significantly upregulated from 12 to 33 h post-inoculation, a period defining the biotrophic phase of infection. Computational analysis of sequences suggested that transcripts were non-protein coding, and this was confirmed by transient expression of FLAG-tagged ORFs in P. infestans.

LanguageEnglish
Pages747-759
Number of pages13
JournalMicrobiology
Volume153
Issue number3
DOIs
Publication statusPublished - 1 Mar 2007

Fingerprint

Phytophthora infestans
Genome
Infection
Complementary DNA
Genes
Solanum tuberosum
Oomycetes
Bacterial Artificial Chromosomes
Open Reading Frames
Sequence Analysis
Real-Time Polymerase Chain Reaction

Keywords

  • bacterial artificial chromosome
  • h post-inoculation
  • high-scoring pair
  • locus control region
  • miRNA
  • microRNA
  • non-coding RNA
  • suppression subtractive hybridization

Cite this

Avrova, Anna O. ; Whisson, Stephen C. ; Pritchard, Leighton ; Venter, Eduard ; De Luca, Sergio ; Hein, Ingo ; Birch, Paul R. J. / A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans. In: Microbiology. 2007 ; Vol. 153, No. 3. pp. 747-759.
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A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans. / Avrova, Anna O.; Whisson, Stephen C.; Pritchard, Leighton; Venter, Eduard; De Luca, Sergio; Hein, Ingo; Birch, Paul R. J.

In: Microbiology, Vol. 153, No. 3, 01.03.2007, p. 747-759.

Research output: Contribution to journalArticle

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