A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics

Valentina Busin, Stewart Burgess, Wenmiao Shu

Research output: Contribution to journalArticle

4 Citations (Scopus)
71 Downloads (Pure)

Abstract

This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.
Original languageEnglish
Pages (from-to)1418–1421
Number of pages4
JournalProcedia Engineering
Volume168
DOIs
Publication statusPublished - 4 Sep 2016
Event30th Anniversary Eurosensors Conference - Budapest Congress Centre, Budapest, Hungary
Duration: 4 Sep 20167 Sep 2016
http://eurosensors.akcongress.com

Fingerprint

Animal Diseases
animal diseases
Assays
Animals
Haptoglobins
haptoglobins
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
assays
Enzymes
acute phase proteins
Acute-Phase Proteins
cattle
sandwiches
production costs
lasers
Lasers
Costs and Cost Analysis
Serum
Proteins

Keywords

  • P_ELISA
  • microfluidics
  • veterinary medicine
  • haptoglobin
  • point-of-care
  • multi-pad paper plate
  • optimisation

Cite this

Busin, Valentina ; Burgess, Stewart ; Shu, Wenmiao. / A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics. In: Procedia Engineering. 2016 ; Vol. 168. pp. 1418–1421.
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A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics. / Busin, Valentina; Burgess, Stewart; Shu, Wenmiao.

In: Procedia Engineering, Vol. 168, 04.09.2016, p. 1418–1421.

Research output: Contribution to journalArticle

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KW - P_ELISA

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KW - veterinary medicine

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