A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics

Valentina Busin; Stewart Burgess; Wenmiao Shu

doi:10.1016/j.proeng.2016.11.402

A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics

Valentina Busin, Stewart Burgess, Wenmiao Shu

Biomedical Engineering

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Abstract

This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

Original language	English
Pages (from-to)	1418–1421
Number of pages	4
Journal	Procedia Engineering
Volume	168
DOIs	https://doi.org/10.1016/j.proeng.2016.11.402
Publication status	Published - 4 Sept 2016
Event	30th Anniversary Eurosensors Conference - Budapest Congress Centre, Budapest, Hungary Duration: 4 Sept 2016 → 7 Sept 2016 http://eurosensors.akcongress.com

Keywords

P_ELISA
microfluidics
veterinary medicine
haptoglobin
point-of-care
multi-pad paper plate
optimisation

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10.1016/j.proeng.2016.11.402

Busin-etal-PE2016-A-novel-multi-pad-paper-plate-based-assays-for-rapid-animal-disease-diagnosticsFinal published version, 455 KBLicence: CC BY-NC-ND 4.0

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title = "A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics",

abstract = "This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.",

keywords = "P_ELISA, microfluidics, veterinary medicine, haptoglobin, point-of-care, multi-pad paper plate, optimisation",

author = "Valentina Busin and Stewart Burgess and Wenmiao Shu",

year = "2016",

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doi = "10.1016/j.proeng.2016.11.402",

language = "English",

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journal = "Procedia Engineering",

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AU - Busin, Valentina

AU - Burgess, Stewart

AU - Shu, Wenmiao

PY - 2016/9/4

Y1 - 2016/9/4

N2 - This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

AB - This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

KW - P_ELISA

KW - microfluidics

KW - veterinary medicine

KW - haptoglobin

KW - point-of-care

KW - multi-pad paper plate

KW - optimisation

UR - http://eurosensors.akcongress.com/

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DO - 10.1016/j.proeng.2016.11.402

M3 - Article

SN - 1877-7058

VL - 168

SP - 1418

EP - 1421

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 30th Anniversary Eurosensors Conference

Y2 - 4 September 2016 through 7 September 2016

ER -

A novel multi-pad paper plate (MP3) based assays for rapid animal disease diagnostics

Abstract

Keywords

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