TY - JOUR
T1 - A novel Ag/carrageenan–gelatin hybrid hydrogel nanocomposite and its biological applications
T2 - preparation and characterization
AU - Muthulakshmi, Lakshmanan
AU - Pavithra, U.
AU - Sivaranjani, V.
AU - Balasubramanian, N.
AU - Sakthivel, Kunnathur Murugesan
AU - Pruncu, Catalin Iulian
PY - 2021/3/31
Y1 - 2021/3/31
N2 - A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed. The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent. Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite. The manufactured composite performances were analysed by UV–visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods. The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed. The antibacterial activity was tested against human pathogens viz. S. agalactiae 1661, S. pyogenes 1210 and E. coli. The bacterial cell wall damage of S. agalactiae 1661 was analyzed by scanning electron microscopy. The cytotoxic assay was performed against the A549 lung cancer cells.
AB - A novel biohybrid hydrogel nanocomposite made of natural polymer carrageenan and gelatin protein were developed. The silver nanoparticles were prepared using the carrageenan polymer as reduction and capping agent. Here, the Ag/Carrageenan was combined with gelatin hydrogel using glutaraldehyde having a cross-link role in order to create the biohybrid hydrogel nanocomposite. The manufactured composite performances were analysed by UV–visible spectroscopy, Fourier Transform infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), Energy dispersive X-ray (EDX) spectroscopy and Transmission Electron Microscopy (TEM) methods. The swelling behaviour of the Ag/Carrageenan-gelatin hybrid hydrogel nanocomposite was also analyzed. The antibacterial activity was tested against human pathogens viz. S. agalactiae 1661, S. pyogenes 1210 and E. coli. The bacterial cell wall damage of S. agalactiae 1661 was analyzed by scanning electron microscopy. The cytotoxic assay was performed against the A549 lung cancer cells.
KW - biocompatibility
KW - carrageenan
KW - polysaccharide
KW - capping agent
KW - hydrogel
UR - https://www.journals.elsevier.com/journal-of-the-mechanical-behavior-of-biomedical-materials
U2 - 10.1016/j.jmbbm.2020.104257
DO - 10.1016/j.jmbbm.2020.104257
M3 - Article
SN - 1751-6161
VL - 115
JO - Journal of the Mechanical Behavior of Biomedical Materials
JF - Journal of the Mechanical Behavior of Biomedical Materials
M1 - 104257
ER -