A new synthesis of alkene-containing minor-groove binders and essential hydrogen bonding in binding to DNA and in antibacterial activity

N.G. Anthony, D. Breen, G. Donoghue, A.I. Khalaf, S.P. Mackay, J.A. Parkinson, C.J. Suckling

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A practical synthesis of alkene-containing minor-groove binders for DNA, related to distamycin, with potential for wide structural diversity is described, based upon the Wittig chemistry of N-alkylpyrrole aldehydes. The compounds prepared have been evaluated for binding to DNA by physical methods (melting temperature and NMR) and for their antibacterial activity. Significantly, it was found that alkenes linking the aryl head group of the minor-groove binder promote strong binding to DNA and high antibacterial activity against Gram-positive bacteria. Conversely, a minor-groove binder containing an alkene located towards the alkylamino tail group has a low affinity for DNA and does not show antibacterial activity. These observations suggest an important role for specific hydrogen bonds in the binding of compounds of this type to DNA, and in their antibacterial activity.
Original languageEnglish
Pages (from-to)1843-1850
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume7
Issue number9
Early online date12 Mar 2009
DOIs
Publication statusPublished - 7 May 2009

Keywords

  • gram positive bacteria
  • ligands
  • derivatives
  • distamycin
  • analogs
  • amide

Fingerprint Dive into the research topics of 'A new synthesis of alkene-containing minor-groove binders and essential hydrogen bonding in binding to DNA and in antibacterial activity'. Together they form a unique fingerprint.

  • Cite this