A new platform for single molecule measurements using the fluorous effect

Marina Santana Vega; Carlos J. Bueno-Alejo; Andrea Taladriz Sender; Amanda K. Chaplin; Chloe Farrow; Alexander Axer; Glenn A. Burley; Cyril Dominguez; Hesna Kara; Vasileious Paschalis; Sumera Tubasum; Ian C. Eperon; Andrew J. Hudson; Alasdair W. Clark

doi:10.1117/12.2647953

A new platform for single molecule measurements using the fluorous effect

Marina Santana Vega, Carlos J. Bueno-Alejo, Andrea Taladriz Sender, Amanda K. Chaplin, Chloe Farrow, Alexander Axer, Glenn A. Burley, Cyril Dominguez, Hesna Kara, Vasileious Paschalis, Sumera Tubasum, Ian C. Eperon, Andrew J. Hudson, Alasdair W. Clark

Research output: Contribution to journal › Conference article › peer-review

Abstract

Irreversible adsorption of biomolecules onto imaging substrates is an impediment to expand the applications of single molecule techniques. Traditional polyethylene glycol (PEG) surfaces are only effective at low concentrations of analytes and their structure prevents their use for interferometric scattering (iSCAT) microscopy. We propose a new platform that virtually eliminates non-specific binding thanks to the omniphobicity of perfluorinated compounds, also known as the fluorous effect. Here, we showcase the anti-fouling properties of these substrates at a single molecule level through iSCAT measurements of a protein mixture. We believe these novel engineered substrates show great promise to study biomachinery processes requiring large analyte concentrations, where other passivation methods are not effective, through iSCAT microscopy and other single molecule techniques.

Original language	English
Article number	123860A
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12386
DOIs	https://doi.org/10.1117/12.2647953
Publication status	Published - 15 Mar 2023
Event	Single Molecule Spectroscopy and Superresolution Imaging XVI 2023 - San Francisco, United States Duration: 28 Jan 2023 → 29 Jan 2023

Keywords

fluorous effect
interferometric scattering microscopy
mass photometry
Single-molecule imaging
surface passivation

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10.1117/12.2647953

https://eprints.gla.ac.uk/310020/

Cite this

Vega, M. S., Bueno-Alejo, C. J., Sender, A. T., Chaplin, A. K., Farrow, C., Axer, A., Burley, G. A., Dominguez, C., Kara, H., Paschalis, V., Tubasum, S., Eperon, I. C., Hudson, A. J., & Clark, A. W. (2023). A new platform for single molecule measurements using the fluorous effect. Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 12386, Article 123860A. https://doi.org/10.1117/12.2647953

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abstract = "Irreversible adsorption of biomolecules onto imaging substrates is an impediment to expand the applications of single molecule techniques. Traditional polyethylene glycol (PEG) surfaces are only effective at low concentrations of analytes and their structure prevents their use for interferometric scattering (iSCAT) microscopy. We propose a new platform that virtually eliminates non-specific binding thanks to the omniphobicity of perfluorinated compounds, also known as the fluorous effect. Here, we showcase the anti-fouling properties of these substrates at a single molecule level through iSCAT measurements of a protein mixture. We believe these novel engineered substrates show great promise to study biomachinery processes requiring large analyte concentrations, where other passivation methods are not effective, through iSCAT microscopy and other single molecule techniques.",

keywords = "fluorous effect, interferometric scattering microscopy, mass photometry, Single-molecule imaging, surface passivation",

author = "Vega, {Marina Santana} and Bueno-Alejo, {Carlos J.} and Sender, {Andrea Taladriz} and Chaplin, {Amanda K.} and Chloe Farrow and Alexander Axer and Burley, {Glenn A.} and Cyril Dominguez and Hesna Kara and Vasileious Paschalis and Sumera Tubasum and Eperon, {Ian C.} and Hudson, {Andrew J.} and Clark, {Alasdair W.}",

year = "2023",

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doi = "10.1117/12.2647953",

language = "English",

volume = "12386",

journal = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

issn = "1605-7422",

note = "Single Molecule Spectroscopy and Superresolution Imaging XVI 2023 ; Conference date: 28-01-2023 Through 29-01-2023",

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Vega, MS, Bueno-Alejo, CJ, Sender, AT, Chaplin, AK, Farrow, C, Axer, A, Burley, GA, Dominguez, C, Kara, H, Paschalis, V, Tubasum, S, Eperon, IC, Hudson, AJ & Clark, AW 2023, 'A new platform for single molecule measurements using the fluorous effect', Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12386, 123860A. https://doi.org/10.1117/12.2647953

TY - JOUR

T1 - A new platform for single molecule measurements using the fluorous effect

AU - Vega, Marina Santana

AU - Bueno-Alejo, Carlos J.

AU - Sender, Andrea Taladriz

AU - Chaplin, Amanda K.

AU - Farrow, Chloe

AU - Axer, Alexander

AU - Burley, Glenn A.

AU - Dominguez, Cyril

AU - Kara, Hesna

AU - Paschalis, Vasileious

AU - Tubasum, Sumera

AU - Eperon, Ian C.

AU - Hudson, Andrew J.

AU - Clark, Alasdair W.

PY - 2023/3/15

Y1 - 2023/3/15

N2 - Irreversible adsorption of biomolecules onto imaging substrates is an impediment to expand the applications of single molecule techniques. Traditional polyethylene glycol (PEG) surfaces are only effective at low concentrations of analytes and their structure prevents their use for interferometric scattering (iSCAT) microscopy. We propose a new platform that virtually eliminates non-specific binding thanks to the omniphobicity of perfluorinated compounds, also known as the fluorous effect. Here, we showcase the anti-fouling properties of these substrates at a single molecule level through iSCAT measurements of a protein mixture. We believe these novel engineered substrates show great promise to study biomachinery processes requiring large analyte concentrations, where other passivation methods are not effective, through iSCAT microscopy and other single molecule techniques.

AB - Irreversible adsorption of biomolecules onto imaging substrates is an impediment to expand the applications of single molecule techniques. Traditional polyethylene glycol (PEG) surfaces are only effective at low concentrations of analytes and their structure prevents their use for interferometric scattering (iSCAT) microscopy. We propose a new platform that virtually eliminates non-specific binding thanks to the omniphobicity of perfluorinated compounds, also known as the fluorous effect. Here, we showcase the anti-fouling properties of these substrates at a single molecule level through iSCAT measurements of a protein mixture. We believe these novel engineered substrates show great promise to study biomachinery processes requiring large analyte concentrations, where other passivation methods are not effective, through iSCAT microscopy and other single molecule techniques.

KW - fluorous effect

KW - interferometric scattering microscopy

KW - mass photometry

KW - Single-molecule imaging

KW - surface passivation

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U2 - 10.1117/12.2647953

DO - 10.1117/12.2647953

M3 - Conference article

AN - SCOPUS:85159772312

SN - 1605-7422

VL - 12386

JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

M1 - 123860A

T2 - Single Molecule Spectroscopy and Superresolution Imaging XVI 2023

Y2 - 28 January 2023 through 29 January 2023

ER -

A new platform for single molecule measurements using the fluorous effect

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Keywords

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