A new device for in vitro evaluation of thrombogenicity

P. Lermusiaux, T.V. How, R.A. Black

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    A device to measure the time of coagulation of whole blood has been designed in order to facilitate testing of thrombogenicity of biomaterials. The principle of operation of the apparatus is to record the time taken for a sphere to fall through a sample of blood. The coagulation time is defined here as the time from collection of the blood sample to coagulation, coagulation having deemed to have occurred when the sphere is prevented from falling by the presence of the fibrin-cell network. The device was tested with homogeneous fluids of different viscosity, milk containing different amounts of rennin and calcium chloride and non anti-coagulated whole blood obtained from five volunteers. Repeat measurements made with each homogeneous fluid show that the variance in the transit time of the sphere is small and consistent with small dispersion. In contrast, the onset of coagulation in milk and blood samples was readily detected. The clotting of milk was highly dependant on the concentration of rennin and calcium chloride. The coagulation time of blood samples from five individuals ranged from 23 to 33 min but simultaneous measurements of samples from the same individual in two identical devices agreed to within ±1 min in all cases. This device may be easily adapted for use in studies to determine the thromboresistance of biomaterials where the onset of coagulation of whole blood in contact with different materials may be readily compared.
    LanguageEnglish
    Pages389-393
    Number of pages4
    JournalMedical Physics
    Volume28
    Issue number4
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Equipment and Supplies
    Chymosin
    Calcium Chloride
    Milk
    Biocompatible Materials
    Blood Coagulation
    Whole Blood Coagulation Time
    Accidental Falls
    Fibrin
    Viscosity
    Volunteers
    In Vitro Techniques

    Keywords

    • thrombosis
    • haemocompatibility
    • coagulation
    • bioengineering

    Cite this

    Lermusiaux, P. ; How, T.V. ; Black, R.A. / A new device for in vitro evaluation of thrombogenicity. In: Medical Physics. 2006 ; Vol. 28, No. 4. pp. 389-393.
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    A new device for in vitro evaluation of thrombogenicity. / Lermusiaux, P.; How, T.V.; Black, R.A.

    In: Medical Physics, Vol. 28, No. 4, 2006, p. 389-393.

    Research output: Contribution to journalArticle

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