A new ceramide along with eight known compounds from the roots of Artemisia incisa pamp

Mamoon-Ur-Rashid, Muhammad Alamzeb, Saqib Ali, Ashfaq Ahmad Khan, John O Igoli, Valerie A Ferro, Alexander Irvine Gray, Mohammad Rafiullah Khan

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4 Citations (Scopus)
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Abstract

A new compound (1) (named as artemceramide-B) together with eight known compounds (taraxerol (2), taraxerol acetate (3), β-sitosterol (4), stigmasterol (5), trans-ethyl caffeate, dracunculin (7), scoparone (8) and isoscopoletin (9) were isolated from an ethanolic extract of the roots of Artemisia incisa Pamp (Asteracae). The structures of the compounds were determined through IR, 1D NMR (1H NMR, 13C NMR) and 2D NMR (COSY, NOESY, HSQC and HMBC) analyses. Accurate mass analyses were done with EI-MS, ESI-MS and acid methanolysis of compound 1 followed by GS-MS studies. The relative stereochemistry of artemceramide-B was determined by comparing its specific rotation and spectroscopic data with the literature. Compounds 1-9 were tested for their anti-bacterial potential against five bacteria strains; Staphylococcus epidermidis, Staphylococcus aureus, Klebsiella pneumoniae, Bacillus subtilis and Escherichia coli. Compound 1 (new) (MIC: 0.0157, 0.0313 mg/mL) and 7 (MIC: 0.0815 , 1.000 mg/mL) showed excellent activities against S. epidermidis and S. aureus while compound 9 showed excellent activities (MIC: 0.0700 , 1.234, 1.890 and 2.286 mg/mL) against S. epidermidis,S. aureus, K. pneumoniae and E. coli, respectively. Compound 6 (MIC: 2.000 mg/mL) was found to be active against E. coli while neither of the compounds showed potential activity against B. subtilis.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalRecords of Natural Products
Volume9
Issue number3
Publication statusPublished - 30 Sep 2015

Keywords

  • A. incisa
  • artemceramide-B
  • asteraceae
  • methanolysis

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    Mamoon-Ur-Rashid, Alamzeb, M., Ali, S., Khan, A. A., Igoli, J. O., Ferro, V. A., Gray, A. I., & Khan, M. R. (2015). A new ceramide along with eight known compounds from the roots of Artemisia incisa pamp. Records of Natural Products, 9(3), 297-304.