A new approach to fluorescence lifetime sensing based on molecular distributions

Research output: Contribution to journalArticle

Abstract

Fluorescence resonance energy transfer (FRET) from donor to acceptor molecules is one of the most powerful techniques for monitoring structure and dynamics. This is because FRET has a strong spatial dependence with angstroms resolution. This dependence includes the simplest case of a random distribution of acceptors for which an analytical solution exists for the fluorescence impulse response I(t). However, in general the acceptor distribution function p(r) is not random and a unique solution cannot be found for I(t). In many important applications of FRET eg in proteins, the simple random treatment is quite inappropriate and yet the information concerning conformation changes is preserved in p(r). One approach, which as been applied to the problem of determining p(r), is to make some assumptions as to its form eg Gaussian and then try to use this to describe I(t).
LanguageEnglish
Pages1-11
Number of pages10
JournalProceedings of SPIE: The International Society for Optical Engineering
Volume4252
Issue number1
DOIs
Publication statusPublished - 2003

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Fluorescence Lifetime
resonance fluorescence
Fluorescence
Energy Transfer
Sensing
energy transfer
life (durability)
fluorescence
Impulse response
statistical distributions
Spatial Dependence
Distribution functions
Conformations
impulses
Impulse Response
distribution functions
Conformation
Unique Solution
proteins
Proteins

Keywords

  • fluorescence
  • resonance
  • energy transfer
  • donor-acceptor
  • distribution function
  • inverse problem
  • lifetime sensing
  • glucose sensor

Cite this

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title = "A new approach to fluorescence lifetime sensing based on molecular distributions",
abstract = "Fluorescence resonance energy transfer (FRET) from donor to acceptor molecules is one of the most powerful techniques for monitoring structure and dynamics. This is because FRET has a strong spatial dependence with angstroms resolution. This dependence includes the simplest case of a random distribution of acceptors for which an analytical solution exists for the fluorescence impulse response I(t). However, in general the acceptor distribution function p(r) is not random and a unique solution cannot be found for I(t). In many important applications of FRET eg in proteins, the simple random treatment is quite inappropriate and yet the information concerning conformation changes is preserved in p(r). One approach, which as been applied to the problem of determining p(r), is to make some assumptions as to its form eg Gaussian and then try to use this to describe I(t).",
keywords = "fluorescence, resonance, energy transfer, donor-acceptor, distribution function, inverse problem, lifetime sensing, glucose sensor",
author = "O.J. Rolinski and D.J.S. Birch and L.J. McCartney and J.C. Pickup",
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T1 - A new approach to fluorescence lifetime sensing based on molecular distributions

AU - Rolinski, O.J.

AU - Birch, D.J.S.

AU - McCartney, L.J.

AU - Pickup, J.C.

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AB - Fluorescence resonance energy transfer (FRET) from donor to acceptor molecules is one of the most powerful techniques for monitoring structure and dynamics. This is because FRET has a strong spatial dependence with angstroms resolution. This dependence includes the simplest case of a random distribution of acceptors for which an analytical solution exists for the fluorescence impulse response I(t). However, in general the acceptor distribution function p(r) is not random and a unique solution cannot be found for I(t). In many important applications of FRET eg in proteins, the simple random treatment is quite inappropriate and yet the information concerning conformation changes is preserved in p(r). One approach, which as been applied to the problem of determining p(r), is to make some assumptions as to its form eg Gaussian and then try to use this to describe I(t).

KW - fluorescence

KW - resonance

KW - energy transfer

KW - donor-acceptor

KW - distribution function

KW - inverse problem

KW - lifetime sensing

KW - glucose sensor

UR - http://dx.doi.org/10.1117/12.426725

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DO - 10.1117/12.426725

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