A new approach for DNA detection by SERRS

K. Faulds; L. Fruk; D.C. Robson; D.G. Thompson; A. Enright; W.E. Smith; D. Graham

doi:10.1039/b506219e

A new approach for DNA detection by SERRS

K. Faulds, L. Fruk, D.C. Robson, D.G. Thompson, A. Enright, W.E. Smith, D. Graham

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering ( SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.

Original language	English
Pages (from-to)	261-268
Number of pages	7
Journal	Faraday Discussions
Volume	2006
Issue number	132
DOIs	https://doi.org/10.1039/b506219e
Publication status	Published - 2006

Keywords

resonance raman-scattering
surface-enhanced raman
benzotriazole azo-dye
molecular beacon
colloidal silver
labeled dna
oligonucleotides
fluorescence
identification
nanoparticles

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title = "A new approach for DNA detection by SERRS",

abstract = "A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering ( SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.",

keywords = "resonance raman-scattering, surface-enhanced raman, benzotriazole azo-dye, molecular beacon, colloidal silver, labeled dna, oligonucleotides, fluorescence, identification, nanoparticles",

author = "K. Faulds and L. Fruk and D.C. Robson and D.G. Thompson and A. Enright and W.E. Smith and D. Graham",

year = "2006",

doi = "10.1039/b506219e",

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publisher = "Royal Society of Chemistry",

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T1 - A new approach for DNA detection by SERRS

AU - Faulds, K.

AU - Fruk, L.

AU - Robson, D.C.

AU - Thompson, D.G.

AU - Enright, A.

AU - Smith, W.E.

AU - Graham, D.

PY - 2006

Y1 - 2006

N2 - A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering ( SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.

AB - A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering ( SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.

KW - resonance raman-scattering

KW - surface-enhanced raman

KW - benzotriazole azo-dye

KW - molecular beacon

KW - colloidal silver

KW - labeled dna

KW - oligonucleotides

KW - fluorescence

KW - identification

KW - nanoparticles

UR - http://www.rsc.org/ej/FD/2006/b506219e.pdf

UR - http://dx.doi.org/10.1039/b506219e

U2 - 10.1039/b506219e

DO - 10.1039/b506219e

M3 - Article

SN - 1359-6640

VL - 2006

SP - 261

EP - 268

JO - Faraday Discussions

JF - Faraday Discussions

IS - 132

ER -

A new approach for DNA detection by SERRS

Abstract

Keywords

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