The increasing use of nanomaterials for biomedical applications has raised the need for efficient, robust and low-cost high-throughput assessment of nanotoxicity and cell-nanoparticle interactions. Microfluidics provides the tools for high-throughput single-cell functional monitoring, while gold nanorods have unique potential for intracellular tracking and can simultaneously be used as drug carriers. Presented here is a miniaturised platform that integrates these features with a multimodal approach to cell imaging. A microfluidic device allows for trapping of an array of singlecells, followed by the controlled delivery of nanoparticles into the cell array and subsequent real-time multimodal imaging of cellular interactions with functionalised nanoparticles. This system has been successfully used to assess cellnanoparticle interactions at the single-cell level.
|Title of host publication||4th European Conference on Microfluidics, Limerick, 10-12 December 2014|
|Editors||Stéphane Colin, Gian Luca Morini, Jürgen J. Brandner|
|Place of Publication||Paris|
|Number of pages||5|
|Publication status||Published - Dec 2014|
|Event||Microfluidics 2014 - Castletroy Park Hotel, Limerick, Ireland|
Duration: 10 Dec 2014 → 12 Dec 2014
|Period||10/12/14 → 12/12/14|
- nanoparticle-cell interactions
- real-time multimodal imaging
- biomedical applications
- cell imaging
Cunha-Matos, C. A., Millington, O. M., Wark, A. W., & Zagnoni, M. (2014). A microfluidics tool for high-throughput, real-time multimodal imaging of nanoparticle-cell interactions. In S. Colin, G. L. Morini, & J. J. Brandner (Eds.), 4th European Conference on Microfluidics, Limerick, 10-12 December 2014 Paris.