A metabolomics approach to optimization of bioactive peptide production in marine bacterial co-cultures

Benjamin Michelin, Ruangelie Edrada-Ebel

Research output: Contribution to conferencePoster

Abstract

Marine bacteria are a promising source of undiscovered molecules for medical and/or industrial applications. However, in normal laboratory conditions many of their biosynthetic pathways are not active. Cocultures enable us to induce the expression of these novel bioactive secondary metabolites.Two marine bacteria collected from the Scottish coast, Streptomyces sp. and Rhodococcus sp. were grown in a coculture. The Rhodococcus sp. strain has gene clusters that allow it to express nonribosomal peptides that act as Endothelial Lipase (EL) inhibitors. EL is a validated target for the treatment of atherosclerosis and cardiovascular disease.A metabolomics-based approach was used to compare the effects of different growing conditions on the secondary metabolite production in the coculture. The samples were analysed with HPLC- ESI MS and NMR. Processed data was interpreted using multivariate analysis with SIMCA 15 software. MS data was used to cluster samples based on molecular weight, and NMR was used to cluster samples based on their chemical profile.In addition, NMR and MS data fusion was used to validate and improve the model. Dereplication of the metabolites was done against the Dictionary of Natural Products (Taylor & Francis) database to identify novel compounds.
Original languageEnglish
Publication statusPublished - 14 Nov 2018
EventScottish Metabolomics Networking annual conference 2018 -
Duration: 14 Nov 201815 Nov 2018

Conference

ConferenceScottish Metabolomics Networking annual conference 2018
Period14/11/1815/11/18

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Keywords

  • marine bacteria
  • bioactive secondary metabolites
  • biosynthetic pathways

Cite this

Michelin, B., & Edrada-Ebel, R. (2018). A metabolomics approach to optimization of bioactive peptide production in marine bacterial co-cultures. Poster session presented at Scottish Metabolomics Networking annual conference 2018, .