A material-based platform to modulate fibronectin activity and focal adhesion assembly

Frankie A. Vanterpool, Marco Cantini, F. Philipp Seib, Manuel Salmerón-Sánchez

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We present a detailed characterization of fibronectin (FN) adsorption and cell adhesion on poly(ethyl acrylate) (PEA) and poly(methyl acrylate) (PMA), two polymers with very similar physicochemical properties and chemical structure, which differ in one single methyl group in the lateral chain of the polymer. The globular solution conformation of FN was retained following adsorption onto PMA, whereas spontaneous organization of FN into protein (nano) networks occurred on PEA. This distinct distribution of FN at the material interface promoted a different availability, measured via monoclonal antibody binding, of two domains that facilitated integrin binding to FN: FNIII10 (RGD sequence) and FNIII9 (PHSRN synergy sequence). The enhanced exposure of the synergy domain on PEA compared to PMA triggered different focal adhesion assemblies: L929 fibroblasts showed a higher fraction of smaller focal plaques on PMA (40%) than on PEA (20%). Blocking experiments with monoclonal antibodies against FNIII10 (HFN7.1) and FNIII9 (mAb1937) confirmed the ability of these polymeric substrates to modulate FN conformation. Overall, we propose a simple and versatile material platform that can be used to tune the presentation of a main extracellular matrix protein (FN) to cells, for applications than span from tissue engineering to disease biology.
LanguageEnglish
Pages286-296
Number of pages11
JournalBioResearch Open Access
Volume3
Issue number6
Early online date6 Nov 2014
DOIs
Publication statusPublished - Dec 2014

Fingerprint

Focal Adhesions
Fibronectins
Adhesion
Adsorption
Conformations
Polymers
Monoclonal Antibodies
Extracellular Matrix Proteins
Cell adhesion
Fibroblasts
Tissue Engineering
Tissue engineering
Cell Adhesion
Integrins
Availability
methyl acrylate
Substrates

Keywords

  • fibronectin
  • cell adhesion
  • monoclonal antibody binding

Cite this

Vanterpool, Frankie A. ; Cantini, Marco ; Seib, F. Philipp ; Salmerón-Sánchez, Manuel. / A material-based platform to modulate fibronectin activity and focal adhesion assembly. In: BioResearch Open Access. 2014 ; Vol. 3, No. 6. pp. 286-296.
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A material-based platform to modulate fibronectin activity and focal adhesion assembly. / Vanterpool, Frankie A.; Cantini, Marco; Seib, F. Philipp; Salmerón-Sánchez, Manuel.

In: BioResearch Open Access, Vol. 3, No. 6, 12.2014, p. 286-296.

Research output: Contribution to journalArticle

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