TY - GEN
T1 - A lung-on-chip to measure oxygen affinity of single red blood cells
AU - Di Caprio, G.
AU - Shaak, D.
AU - Higgins, J. M.
AU - Schonbrun, E.
PY - 2014/10/30
Y1 - 2014/10/30
N2 - We have developed a functional cytometry measurement system that quantifies volume, hemoglobin mass and oxygen saturation for individual red blood cells (RBCs) in high throughput. Cells are measured in a microfluidic device that mimics the alveolar-capillary barrier of the lung, enabling precise control of oxygen partial pressure. By evaluating the oxygen saturation of individual cells, we can quantify the variability of oxygen affinity across a red blood cell population. Because both glycated and fetal hemoglobin have a different oxygen affinity than adult hemoglobin, this variability is critical in understanding the hematological response to both diabetes and sickle cell anemia.
AB - We have developed a functional cytometry measurement system that quantifies volume, hemoglobin mass and oxygen saturation for individual red blood cells (RBCs) in high throughput. Cells are measured in a microfluidic device that mimics the alveolar-capillary barrier of the lung, enabling precise control of oxygen partial pressure. By evaluating the oxygen saturation of individual cells, we can quantify the variability of oxygen affinity across a red blood cell population. Because both glycated and fetal hemoglobin have a different oxygen affinity than adult hemoglobin, this variability is critical in understanding the hematological response to both diabetes and sickle cell anemia.
KW - absorption spectroscopy
KW - imaging flow cytometry
KW - organ-on-chip
KW - red blood cells
UR - http://www.scopus.com/inward/record.url?scp=84941622246&partnerID=8YFLogxK
M3 - Conference contribution book
AN - SCOPUS:84941622246
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 9
EP - 11
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CY - San Diego, California
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -
