A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the
development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.
Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)
Pages946-948
Number of pages3
Publication statusPublished - 2013

Fingerprint

Microfluidics
Throughput
Lab-on-a-chip
Liposomes
Phospholipids
Metabolites
Biosensors
Assays
Screening
Automation
Fluorescence
Proteins
Membranes
Costs
Water

Keywords

  • microfluidics
  • lab-on-a-chip
  • passive channel networks
  • Droplet-Interface-Bilayers
  • DIBs

Cite this

Schlicht, B., & Zagnoni, M. (2013). A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013) (pp. 946-948)
Schlicht, Barbara ; Zagnoni, Michele. / A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013). 2013. pp. 946-948
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Schlicht, B & Zagnoni, M 2013, A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013). pp. 946-948.

A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. / Schlicht, Barbara; Zagnoni, Michele.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013). 2013. p. 946-948.

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

TY - CHAP

T1 - A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

AU - Schlicht, Barbara

AU - Zagnoni, Michele

PY - 2013

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N2 - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

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KW - microfluidics

KW - lab-on-a-chip

KW - passive channel networks

KW - Droplet-Interface-Bilayers

KW - DIBs

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M3 - Chapter (peer-reviewed)

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BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

ER -

Schlicht B, Zagnoni M. A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013). 2013. p. 946-948