Abstract
| Language | English |
|---|---|
| Number of pages | 3 |
| Publication status | Published - 31 Oct 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 http://microtasconferences.org/microtas2013/ |
Conference
| Conference | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013 |
|---|---|
| Abbreviated title | MicroTAS 2013 |
| Country | Germany |
| City | Freiburg |
| Period | 27/10/13 → 31/10/13 |
| Internet address |
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Keywords
- microfluidics
- lab - on - a - chip
- passive channel networks
- droplet - interface - bilayers
Cite this
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A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. / Schlicht, Barbara; Zagnoni, Michele.
2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.Research output: Contribution to conference › Proceeding
TY - CONF
T1 - A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays
AU - Schlicht, Barbara
AU - Zagnoni, Michele
PY - 2013/10/31
Y1 - 2013/10/31
N2 - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.
AB - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.
KW - microfluidics
KW - lab - on - a - chip
KW - passive channel networks
KW - droplet - interface - bilayers
M3 - Proceeding
ER -