A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

Barbara Schlicht; Michele Zagnoni

A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

Electronic And Electrical Engineering

Research output: Contribution to conference › Proceeding

Abstract

The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

Original language	English
Number of pages	3
Publication status	Published - 31 Oct 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013 - Freiburg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 http://microtasconferences.org/microtas2013/

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013
Abbreviated title	MicroTAS 2013
Country	Germany
City	Freiburg
Period	27/10/13 → 31/10/13
Internet address	http://microtasconferences.org/microtas2013/

Keywords

microfluidics
lab - on - a - chip
passive channel networks
droplet - interface - bilayers

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Projects

1 Finished

EPSRC DOCTORAL TRAINING GRANT | Schlicht, Barbara

Zagnoni, M., Uttamchandani, D. & Schlicht, B.

EPSRC (Engineering and Physical Sciences Research Council)

1/10/11 → 25/09/19

Project: Research Studentship - Internally Allocated

Cite this

Schlicht, B., & Zagnoni, M. (2013). A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.

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AU - Zagnoni, Michele

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N2 - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

AB - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

KW - microfluidics

KW - lab - on - a - chip

KW - passive channel networks

KW - droplet - interface - bilayers

M3 - Proceeding

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013

Y2 - 27 October 2013 through 31 October 2013

ER -