A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

Research output: Contribution to conferenceProceeding

Abstract

The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013
Abbreviated titleMicroTAS 2013
CountryGermany
CityFreiburg
Period27/10/1331/10/13
Internet address

Fingerprint

Microfluidics
Throughput
Lab-on-a-chip
Liposomes
Phospholipids
Metabolites
Biosensors
Assays
Screening
Automation
Fluorescence
Proteins
Membranes
Costs
Water

Keywords

  • microfluidics
  • lab - on - a - chip
  • passive channel networks
  • droplet - interface - bilayers

Cite this

Schlicht, B., & Zagnoni, M. (2013). A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.
Schlicht, Barbara ; Zagnoni, Michele. / A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.3 p.
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abstract = "The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.",
keywords = "microfluidics, lab - on - a - chip, passive channel networks, droplet - interface - bilayers",
author = "Barbara Schlicht and Michele Zagnoni",
year = "2013",
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Schlicht, B & Zagnoni, M 2013, 'A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays' 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany, 27/10/13 - 31/10/13, .

A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. / Schlicht, Barbara; Zagnoni, Michele.

2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.

Research output: Contribution to conferenceProceeding

TY - CONF

T1 - A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays

AU - Schlicht, Barbara

AU - Zagnoni, Michele

PY - 2013/10/31

Y1 - 2013/10/31

N2 - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

AB - The ability to form artificial lipid membranes in a reliable, high-throughput lab-on-a-chip format has the potential to advance membrane protein studies and the development of sensitive molecular biosensors, ultimately impacting on the development of novel and low-cost synthetic approaches to drug screening. Existing methods are limited in terms of their automation, throughput and ease of use. We have developed a microfluidic system that allows the formation, alternation, desired positioning and long-term storage of arrays of droplet-interface-bilayers (DIBs). By encapsulating the desired cocktail of liposomes and metabolites into phospholipid-stabilized water-in-oil (W/O) droplets, hundreds of DIBs were characterized using fluorescence-based assays.

KW - microfluidics

KW - lab - on - a - chip

KW - passive channel networks

KW - droplet - interface - bilayers

M3 - Proceeding

ER -

Schlicht B, Zagnoni M. A high-throughput microfluidic system for the simultaneous formation of droplet-interface bilayer arrays. 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - MicroTAS 2013, Freiburg, Germany.