A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammation

Clett Erridge, T. Attina, C.M. Spickett, David J. Webb

Research output: Contribution to journalArticle

427 Citations (Scopus)

Abstract

Bacterial endotoxin is a potently inflammatory antigen that is abundant in the human gut. Endotoxin circulates at low concentrations in the blood of all healthy individuals, although elevated concentrations are associated with an increased risk of atherosclerosis. We sought to determine whether a high-fat meal or smoking increases plasma endotoxin concentrations and whether such concentrations are of physiologic relevance. Plasma endotoxin and endotoxin neutralization capacity were measured for 4 h in 12 healthy men after no meal, 3 cigarettes, a high-fat meal, or a high-fat meal with 3 cigarettes by using the limulus assay. Baseline endotoxin concentrations were 8.2 pg/mL (interquartile range: 3.4-13.5 pg/mL) but increased significantly (P < 0.05) by 50% after a high-fat meal or after a high-fat meal with cigarettes but not after no meal or cigarettes alone. These results were validated by the observations that a high-fat meal with or without cigarettes, but not no meal or smoking, also significantly (P < 0.05) reduced plasma endotoxin neutralization capacity, which is an indirect measure of endotoxin exposure. Human monocytes, but not aortic endothelial cells, were responsive to transient (30 s) or low-dose (10 pg/mL) exposure to endotoxin. However, plasma from whole blood treated with as little as 10 pg endotoxin/mL increased the endothelial cell expression of E-selectin, at least partly via tumor necrosis factor--induced cellular activation. Low-grade endotoxemia may contribute to the postprandial inflammatory state and could represent a novel potential contributor to endothelial activation and the development of atherosclerosis.
LanguageEnglish
Pages1286-1292
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Volume86
Issue number5
Publication statusPublished - Nov 2007

Fingerprint

Endotoxemia
Endotoxins
Meals
Fats
Inflammation
Tobacco Products
Atherosclerosis
Endothelial Cells
Smoking
Horseshoe Crabs
E-Selectin
Monocytes
Tumor Necrosis Factor-alpha
Antigens

Keywords

  • atherosclerosis
  • endotoxin
  • inflammation.
  • diet
  • postprandial lipemia
  • smoking
  • blood mononuclear-cells
  • factor-kappa-b
  • nonalcoholic steatohepatitis
  • carotid atherosclerosis
  • neutralizing-capacity
  • insulin-resistance
  • healthy-volunteers
  • cytokine release
  • olive oil
  • activation

Cite this

Erridge, Clett ; Attina, T. ; Spickett, C.M. ; Webb, David J. / A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammation. In: American Journal of Clinical Nutrition. 2007 ; Vol. 86, No. 5. pp. 1286-1292.
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A high-fat meal induces low-grade endotoxemia: evidence of a novel mechanism of postprandial inflammation. / Erridge, Clett; Attina, T.; Spickett, C.M.; Webb, David J.

In: American Journal of Clinical Nutrition, Vol. 86, No. 5, 11.2007, p. 1286-1292.

Research output: Contribution to journalArticle

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AU - Erridge, Clett

AU - Attina, T.

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KW - insulin-resistance

KW - healthy-volunteers

KW - cytokine release

KW - olive oil

KW - activation

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