Abstract
In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.
| Original language | English |
|---|---|
| Pages (from-to) | 497-509 |
| Number of pages | 13 |
| Journal | Journal of Theoretical Biology |
| Volume | 234 |
| Issue number | 4 |
| Early online date | 29 Jan 2005 |
| DOIs | |
| Publication status | Published - 21 Jun 2005 |
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Keywords
- error-prone
- model
- PCR
- polymerase chain reaction
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A general model of error-prone PCR. / Pritchard, Leighton; Corne, Dave; Kell, Douglas; Rowland, Jem; Winson, Mike.
In: Journal of Theoretical Biology, Vol. 234, No. 4, 21.06.2005, p. 497-509.Research output: Contribution to journal › Article
TY - JOUR
T1 - A general model of error-prone PCR
AU - Pritchard, Leighton
AU - Corne, Dave
AU - Kell, Douglas
AU - Rowland, Jem
AU - Winson, Mike
PY - 2005/6/21
Y1 - 2005/6/21
N2 - In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.
AB - In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.
KW - error-prone
KW - model
KW - PCR
KW - polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=16244419795&partnerID=8YFLogxK
U2 - 10.1016/j.jtbi.2004.12.005
DO - 10.1016/j.jtbi.2004.12.005
M3 - Article
C2 - 15808871
AN - SCOPUS:16244419795
VL - 234
SP - 497
EP - 509
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
SN - 0022-5193
IS - 4
ER -