A general model of error-prone PCR

Leighton Pritchard, Dave Corne, Douglas Kell, Jem Rowland, Mike Winson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In this paper, we generalise a previously-described model of the error-prone polymerase chain reaction (PCR) reaction to conditions of arbitrarily variable amplification efficiency and initial population size. Generalisation of the model to these conditions improves the correspondence to observed and expected behaviours of PCR, and restricts the extent to which the model may explore sequence space for a prescribed set of parameters. Error-prone PCR in realistic reaction conditions is predicted to be less effective at generating grossly divergent sequences than the original model. The estimate of mutation rate per cycle by sampling sequences from an in vitro PCR experiment is correspondingly affected by the choice of model and parameters.

LanguageEnglish
Pages497-509
Number of pages13
JournalJournal of Theoretical Biology
Volume234
Issue number4
Early online date29 Jan 2005
DOIs
Publication statusPublished - 21 Jun 2005

Fingerprint

Polymerase Chain Reaction
Polymerase chain reaction
polymerase chain reaction
Mutation Rate
Population Density
Model
Sequence Space
Population Size
Amplification
Mutation
population size
Correspondence
Sampling
mutation
Cycle
Generalise
Estimate
Experiment
Experiments
sampling

Keywords

  • error-prone
  • model
  • PCR
  • polymerase chain reaction

Cite this

Pritchard, Leighton ; Corne, Dave ; Kell, Douglas ; Rowland, Jem ; Winson, Mike. / A general model of error-prone PCR. In: Journal of Theoretical Biology. 2005 ; Vol. 234, No. 4. pp. 497-509.
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Pritchard, L, Corne, D, Kell, D, Rowland, J & Winson, M 2005, 'A general model of error-prone PCR' Journal of Theoretical Biology, vol. 234, no. 4, pp. 497-509. https://doi.org/10.1016/j.jtbi.2004.12.005

A general model of error-prone PCR. / Pritchard, Leighton; Corne, Dave; Kell, Douglas; Rowland, Jem; Winson, Mike.

In: Journal of Theoretical Biology, Vol. 234, No. 4, 21.06.2005, p. 497-509.

Research output: Contribution to journalArticle

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