A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

Tania Saxl, Faaizah Khan, Matteo Ferla, David Birch, John Pickup

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP-Badan-beads. Either GBP-Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent K-d of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.

LanguageEnglish
Pages968-972
Number of pages5
JournalAnalyst
Volume136
Issue number5
Early online date17 Dec 2010
DOIs
Publication statusPublished - 2011

Fingerprint

Glucose sensors
fiber optics
Fiber optic sensors
Glucose
glucose
fluorescence
Fluorescence
sensor
Polystyrenes
protein
Sepharose
Nitrilotriacetic Acid
Laser excitation
Multimode fibers
Fluorophores
Medical problems
Photodiodes
Pulsed lasers
Biosensors
Optical Fibers

Keywords

  • resonance-energy transfer
  • in-vivo
  • biosensor
  • micocapsules
  • assay
  • TIC - Bionanotechnology

Cite this

Saxl, Tania ; Khan, Faaizah ; Ferla, Matteo ; Birch, David ; Pickup, John. / A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein. In: Analyst. 2011 ; Vol. 136, No. 5. pp. 968-972.
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A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein. / Saxl, Tania; Khan, Faaizah; Ferla, Matteo; Birch, David; Pickup, John.

In: Analyst, Vol. 136, No. 5, 2011, p. 968-972.

Research output: Contribution to journalArticle

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T1 - A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

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