A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein

Tania Saxl, Faaizah Khan, Matteo Ferla, David Birch, John Pickup

Research output: Contribution to journalArticle

40 Citations (Scopus)
252 Downloads (Pure)

Abstract

Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Fluorescence lifetime increased in response to glucose, observed by fluorescence lifetime imaging microscopy of the GBP-Badan-beads. Either GBP-Badan agarose or polystyrene beads were loaded into a porous chamber at the end of a multimode optical fibre. Fluorescence lifetime responses were recorded using pulsed laser excitation, high speed photodiode detection and time-correlated single-photon counting. The maximal response was at 100 mM glucose with an apparent K-d of 13 mM (agarose) and 20 mM (polystyrene), and good working-day stability was demonstrated. We conclude that fluorescence lifetime fibre-optic glucose sensors based on GBP-Badan are suitable for development as clinical glucose monitors.

Original languageEnglish
Pages (from-to)968-972
Number of pages5
JournalAnalyst
Volume136
Issue number5
Early online date17 Dec 2010
DOIs
Publication statusPublished - 2011

Keywords

  • resonance-energy transfer
  • in-vivo
  • biosensor
  • micocapsules
  • assay
  • TIC - Bionanotechnology

Fingerprint Dive into the research topics of 'A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein'. Together they form a unique fingerprint.

Cite this