A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

J. Trägårdh, J. Schniete, M. Parsons, G. McConnell

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Abstract

We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.
Original languageEnglish
Article number091303
Number of pages5
JournalAPL Photonics
Volume1
Issue number9
Early online date27 Sep 2016
DOIs
Publication statusPublished - 31 Dec 2016

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Keywords

  • Raman
  • lasers
  • microscopy
  • live cell
  • multi-photon
  • harmonic generation

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