A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

J. Trägårdh; J. Schniete; M. Parsons; G. McConnell

doi:10.1063/1.4962207

A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

J. Trägårdh, J. Schniete, M. Parsons, G. McConnell

Strathclyde Institute Of Pharmacy And Biomedical Sciences

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Abstract

We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.

Original language	English
Article number	091303
Number of pages	5
Journal	APL Photonics
Volume	1
Issue number	9
Early online date	27 Sept 2016
DOIs	https://doi.org/10.1063/1.4962207
Publication status	Published - 31 Dec 2016

Keywords

Raman
lasers
microscopy
live cell
multi-photon
harmonic generation

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10.1063/1.4962207Licence: CC BY 4.0

Tragardh-etal-APLP2016-a-femtosecond-raman-generatorFinal published version, 3.35 MBLicence: CC BY 4.0

Cite this

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abstract = "We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.",

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AU - Trägårdh, J.

AU - Schniete, J.

AU - Parsons, M.

AU - McConnell, G.

PY - 2016/12/31

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N2 - We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.

AB - We demonstrate a femtosecond single pass Raman generator based on an YVO crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broad band spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein (RFP) expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.

KW - Raman

KW - lasers

KW - microscopy

KW - live cell

KW - multi-photon

KW - harmonic generation

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ER -

A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

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Keywords

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MESOLAB: A Centre For Optical Mesoscopy For Biomedical Research At The University Of Strathclyde

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A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

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MESOLAB: A Centre For Optical Mesoscopy For Biomedical Research At The University Of Strathclyde

Cite this