Abstract
We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).
| Original language | English |
|---|---|
| Pages (from-to) | 3298-3303 |
| Number of pages | 6 |
| Journal | Chemical Science |
| Volume | 7 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 5 Feb 2016 |
Funding
We are thankful for the support given by the Spanish grants SAF2013-41943-R, CTQ2013-43264-R and CTQ2013-49317-EXP, the Xunta de Galicia GRC2013-041, the ERDF, and the European Research Council (Advanced Grant No. 340055). Support of COST Action CM1105, COST CM1306 and the orfeo-cinqa network are kindly acknowledged. J. R. thanks the Xunta de Galicia for her PhD fellowship. All calculations were carried out at the CESGA. We thank Dr Jo? Couceiro for his help with the cell internalization experiments.
Keywords
- DNA
- DNA-binding peptide
- chimera
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