A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Jéssica Rodríguez, Jesús Mosquera, Rebeca García-Fandiño, M. Eugenio Vázquez, José L. Mascareñas

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

Original languageEnglish
Pages (from-to)3298-3303
Number of pages6
JournalChemical Science
Volume7
Issue number5
DOIs
Publication statusPublished - 5 Feb 2016

Keywords

  • DNA
  • DNA-binding peptide
  • chimera

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    Rodríguez, J., Mosquera, J., García-Fandiño, R., Eugenio Vázquez, M., & Mascareñas, J. L. (2016). A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves. Chemical Science, 7(5), 3298-3303. https://doi.org/10.1039/c6sc00045b