Abstract
We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).
Original language | English |
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Pages (from-to) | 3298-3303 |
Number of pages | 6 |
Journal | Chemical Science |
Volume | 7 |
Issue number | 5 |
DOIs | |
Publication status | Published - 5 Feb 2016 |
Keywords
- DNA
- DNA-binding peptide
- chimera