A demonstration of the effectiveness of a single aberration correction per optical slice in beam scanned optically sectioning microscopes

S. P. Poland, A. J. Wright, S. Cobb, J. C. Vijverberg, J. M. Girkin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this paper we report the use of adaptive optics to correct for sample induced aberrations in optical microscopy, crucially comparing individual pixel-by-pixel correction against a single correction for an entire optical section. Sample induced optical aberrations in slices of rat brain tissue were corrected with a deformable membrane mirror. Using axial resolution measurements, we demonstrate that a single aberration correction per optical slice achieves around 80% of the maximum possible improvement compared to individual pixel-by-pixel correction in both confocal and multiphoton microscopy. A single aberration correction per depth, compared to pixel-by-pixel aberration correction, significantly decreases scan times and therefore reduces photobleaching and phototoxic effects enabling more rapid microscopy with active aberration correction. The results confirm that the use of a “look-up” table, based upon sample type and depth, may be the most practical way of implementing adaptive optic aberration correction in beam scanning optical sectioning microscopy.
Original languageEnglish
Pages (from-to)318-323
Number of pages6
JournalMicron
Volume42
Issue number4
DOIs
Publication statusPublished - Jun 2011

    Fingerprint

Keywords

  • biological imaging
  • implementation
  • system
  • confocal microscopy
  • adaptive optics
  • multiphoton microscopy
  • aberration correction

Cite this