A correlation of FTIR spectra derived from prostate cancer biopsies with Gleason grade and tumour stage

Ehsan Gazi, Matthew Baker, John Dwyer, Nicholas P. Lockyer, Peter Gardner, Jonathan H. Shanks, Roy S. Reeve, Claire A. Hart, Noel W. Clarke, Michael D. Brown

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We introduce biochemistry as a second dimension to Gleason grading, using Fourier transform infrared (FTIR) microspectroscopy. For the first time, we correlate FTIR spectra derived from prostate cancer (pCA) tissue with Gleason score and the clinical stage of the tumour at time of biopsy. Serial sections from paraffin-embedded pCA tissue were collected. One was stained with hematoxylin and eosin and Gleason scored; FTIR spectra were collected from malignant locations using a second unstained section. FTIR spectra, representing different Gleason grades, were used to construct a diagnostic classifier for pCA using linear discriminant analysis (LDA). This model was blind tested using 383 IR spectra from 36 biopsies. Using a three-band Gleason criteria, we obtained sensitivity of ≥70% for the FTIR-LDA model to predict Gleason <7, = 7, and >7, with specificities of ≥81%. Using a threshold of Gleason/FTIR-LDA score of ≥8, we obtained a sensitivity and specificity of 71% and 67%, respectively, for the correlation with metastatic tumours using the FTIR-LDA system and 85% and 63%, respectively, for the correlation of metastatic tumours using the Gleason system. There is a correlation between tissue architecture using Gleason score with tissue biochemistry using FTIR-LDA. Both systems are similar in their performance in predicting metastatic behaviour in tumours from individual patients.

Original languageEnglish
Pages (from-to)750-761
Number of pages12
JournalEuropean Urology
Volume50
Issue number4
Early online date31 Mar 2006
DOIs
Publication statusPublished - 1 Oct 2006

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Fourier Analysis
Prostatic Neoplasms
Discriminant Analysis
Biopsy
Neoplasm Grading
Neoplasms
Biochemistry
Hematoxylin
Eosine Yellowish-(YS)
Paraffin
Sensitivity and Specificity

Keywords

  • FTIR microspectroscopy
  • Gleason grade
  • molecular diagnosis
  • multivariate analysis
  • prostate cancer
  • tumour stage

Cite this

Gazi, Ehsan ; Baker, Matthew ; Dwyer, John ; Lockyer, Nicholas P. ; Gardner, Peter ; Shanks, Jonathan H. ; Reeve, Roy S. ; Hart, Claire A. ; Clarke, Noel W. ; Brown, Michael D. / A correlation of FTIR spectra derived from prostate cancer biopsies with Gleason grade and tumour stage. In: European Urology. 2006 ; Vol. 50, No. 4. pp. 750-761.
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Gazi, E, Baker, M, Dwyer, J, Lockyer, NP, Gardner, P, Shanks, JH, Reeve, RS, Hart, CA, Clarke, NW & Brown, MD 2006, 'A correlation of FTIR spectra derived from prostate cancer biopsies with Gleason grade and tumour stage', European Urology, vol. 50, no. 4, pp. 750-761. https://doi.org/10.1016/j.eururo.2006.03.031

A correlation of FTIR spectra derived from prostate cancer biopsies with Gleason grade and tumour stage. / Gazi, Ehsan; Baker, Matthew; Dwyer, John; Lockyer, Nicholas P.; Gardner, Peter; Shanks, Jonathan H.; Reeve, Roy S.; Hart, Claire A.; Clarke, Noel W.; Brown, Michael D.

In: European Urology, Vol. 50, No. 4, 01.10.2006, p. 750-761.

Research output: Contribution to journalArticle

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AU - Gazi, Ehsan

AU - Baker, Matthew

AU - Dwyer, John

AU - Lockyer, Nicholas P.

AU - Gardner, Peter

AU - Shanks, Jonathan H.

AU - Reeve, Roy S.

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AU - Clarke, Noel W.

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KW - prostate cancer

KW - tumour stage

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