A computational method for two-dimensional quantitative analysis of standing wave images of red blood cells

Research output: Contribution to conferencePoster

Abstract

Standing wave (SW) microscopy allows for an improvement in the axial resolution that can be obtained in optical microscopy. In SW microscopy two counter propagating waves interfere producing a SW with anti-nodal planes that are separated by λ/2n and a FWHM of λ/4n which is the axial resolution, where λ is the excitation wavelength and n is the refractive index Multi-planar SW microscopy, with the addition of a mirror below the specimen, allows for selective plane contour mapping of one concave surface of a red blood cell (RBC). We report a computational method to extract SW anti-nodal plane and boundaries positions (x,y) and extract each pixel intensity value. By doing so, we can create 2D reconstruction of SW RBC images captured at video rate. In future, by utilising the positional information for the each of the anti-nodal SW planes, we aim to create 3D and 4D reconstruction of the RBCs concave surface. Additionally, applying the extended computational method to SW RBC images we aim to study healthy and diseased erythrocytes and the changes in the concave surface morphology over time.

Conference

ConferenceMicroscience Microscopy Congress 2017
CountryUnited Kingdom
CityManchester
Period3/07/176/07/17

Fingerprint

erythrocytes
standing waves
quantitative analysis
Microscopy
Erythrocytes
microscopy
Refractometry
counters
pixels
refractivity
mirrors
wavelengths
excitation

Keywords

  • standing waves
  • mulitplanar excitation
  • 2D reconstruction
  • microscopy

Cite this

@conference{4ef28791522843ddb0f7e33bd40a2306,
title = "A computational method for two-dimensional quantitative analysis of standing wave images of red blood cells",
abstract = "Standing wave (SW) microscopy allows for an improvement in the axial resolution that can be obtained in optical microscopy. In SW microscopy two counter propagating waves interfere producing a SW with anti-nodal planes that are separated by λ/2n and a FWHM of λ/4n which is the axial resolution, where λ is the excitation wavelength and n is the refractive index Multi-planar SW microscopy, with the addition of a mirror below the specimen, allows for selective plane contour mapping of one concave surface of a red blood cell (RBC). We report a computational method to extract SW anti-nodal plane and boundaries positions (x,y) and extract each pixel intensity value. By doing so, we can create 2D reconstruction of SW RBC images captured at video rate. In future, by utilising the positional information for the each of the anti-nodal SW planes, we aim to create 3D and 4D reconstruction of the RBCs concave surface. Additionally, applying the extended computational method to SW RBC images we aim to study healthy and diseased erythrocytes and the changes in the concave surface morphology over time.",
keywords = "standing waves , mulitplanar excitation, 2D reconstruction, microscopy",
author = "Ross Scrimgeour and Tinning, {Peter William} and David Li and Gail McConnell",
year = "2017",
month = "7",
day = "4",
language = "English",
note = "Microscience Microscopy Congress 2017 ; Conference date: 03-07-2017 Through 06-07-2017",

}

A computational method for two-dimensional quantitative analysis of standing wave images of red blood cells. / Scrimgeour, Ross; Tinning, Peter William; Li, David; McConnell, Gail.

2017. Poster session presented at Microscience Microscopy Congress 2017, Manchester, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - A computational method for two-dimensional quantitative analysis of standing wave images of red blood cells

AU - Scrimgeour, Ross

AU - Tinning, Peter William

AU - Li, David

AU - McConnell, Gail

PY - 2017/7/4

Y1 - 2017/7/4

N2 - Standing wave (SW) microscopy allows for an improvement in the axial resolution that can be obtained in optical microscopy. In SW microscopy two counter propagating waves interfere producing a SW with anti-nodal planes that are separated by λ/2n and a FWHM of λ/4n which is the axial resolution, where λ is the excitation wavelength and n is the refractive index Multi-planar SW microscopy, with the addition of a mirror below the specimen, allows for selective plane contour mapping of one concave surface of a red blood cell (RBC). We report a computational method to extract SW anti-nodal plane and boundaries positions (x,y) and extract each pixel intensity value. By doing so, we can create 2D reconstruction of SW RBC images captured at video rate. In future, by utilising the positional information for the each of the anti-nodal SW planes, we aim to create 3D and 4D reconstruction of the RBCs concave surface. Additionally, applying the extended computational method to SW RBC images we aim to study healthy and diseased erythrocytes and the changes in the concave surface morphology over time.

AB - Standing wave (SW) microscopy allows for an improvement in the axial resolution that can be obtained in optical microscopy. In SW microscopy two counter propagating waves interfere producing a SW with anti-nodal planes that are separated by λ/2n and a FWHM of λ/4n which is the axial resolution, where λ is the excitation wavelength and n is the refractive index Multi-planar SW microscopy, with the addition of a mirror below the specimen, allows for selective plane contour mapping of one concave surface of a red blood cell (RBC). We report a computational method to extract SW anti-nodal plane and boundaries positions (x,y) and extract each pixel intensity value. By doing so, we can create 2D reconstruction of SW RBC images captured at video rate. In future, by utilising the positional information for the each of the anti-nodal SW planes, we aim to create 3D and 4D reconstruction of the RBCs concave surface. Additionally, applying the extended computational method to SW RBC images we aim to study healthy and diseased erythrocytes and the changes in the concave surface morphology over time.

KW - standing waves

KW - mulitplanar excitation

KW - 2D reconstruction

KW - microscopy

UR - http://www.mmc-series.org.uk/

M3 - Poster

ER -

Scrimgeour R, Tinning PW, Li D, McConnell G. A computational method for two-dimensional quantitative analysis of standing wave images of red blood cells. 2017. Poster session presented at Microscience Microscopy Congress 2017, Manchester, United Kingdom.