A comparison study of the degradative effects and safety implications of UVC and 405 nm germicidal light sources for endoscope storage

Daniel Irving, Dimitrios A. Lamprou, Michelle MacLean, Scott J. MacGregor, John G. Anderson, M. Helen Grant

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Abstract

Storage of flexible endoscopes under germicidal ultraviolet (UVC) light has been associated with degradation of device material leading to failure and increased risk to patients. 405 nm germicidal light presents a possible alternative, potentially providing effective bacterial inactivation without material damage. Samples of endoscope material were exposed to UVC and 405 nm germicidal light sources and a broad spectrum light source control. Material properties were monitored using FTIR, AFM, contact angle and confocal microscopy. Significant changes were observed with samples exposed to the UVC source, with variations in FTIR spectra indicative of side chain scission, a decrease in contact angle from 82.6° to 61.4°, an increase in average surface roughness from 2.34 nm to 68.7 nm and visible cracking of the surface. In contrast samples exposed to the 405 nm light source showed little to no changes, with any variations being comparable to those seen on samples exposed to the broad spectrum control. Bacterial adhesion tests on samples showed an 86.8% increase in Pseudomonas aeruginosa adhesion on UVC exposed samples and no significant increase in adhesion with samples exposed to the other light sources. 405 nm germicidal light therefore potentially represents a safer alternative to UVC light for use in flexible endoscope storage.
Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalPolymer Degradation and Stability
Volume133
Early online date7 Sep 2016
DOIs
Publication statusPublished - 30 Nov 2016

Fingerprint

endoscopes
Endoscopy
Light sources
safety
light sources
Adhesion
Contact angle
Confocal microscopy
adhesion
Materials properties
adhesion tests
Surface roughness
pseudomonas
Degradation
ultraviolet radiation
deactivation
cleavage
surface roughness
atomic force microscopy
degradation

Keywords

  • germicidal ultraviolet light
  • bacterial inactivation
  • UVC
  • 405 nm light
  • photodegradation
  • bacterial adhesion
  • endoscope material
  • decontamination

Cite this

@article{10bc8a567c2a4562bd8aad97037e3dff,
title = "A comparison study of the degradative effects and safety implications of UVC and 405 nm germicidal light sources for endoscope storage",
abstract = "Storage of flexible endoscopes under germicidal ultraviolet (UVC) light has been associated with degradation of device material leading to failure and increased risk to patients. 405 nm germicidal light presents a possible alternative, potentially providing effective bacterial inactivation without material damage. Samples of endoscope material were exposed to UVC and 405 nm germicidal light sources and a broad spectrum light source control. Material properties were monitored using FTIR, AFM, contact angle and confocal microscopy. Significant changes were observed with samples exposed to the UVC source, with variations in FTIR spectra indicative of side chain scission, a decrease in contact angle from 82.6° to 61.4°, an increase in average surface roughness from 2.34 nm to 68.7 nm and visible cracking of the surface. In contrast samples exposed to the 405 nm light source showed little to no changes, with any variations being comparable to those seen on samples exposed to the broad spectrum control. Bacterial adhesion tests on samples showed an 86.8{\%} increase in Pseudomonas aeruginosa adhesion on UVC exposed samples and no significant increase in adhesion with samples exposed to the other light sources. 405 nm germicidal light therefore potentially represents a safer alternative to UVC light for use in flexible endoscope storage.",
keywords = "germicidal ultraviolet light , bacterial inactivation, UVC, 405 nm light, photodegradation, bacterial adhesion, endoscope material, decontamination",
author = "Daniel Irving and Lamprou, {Dimitrios A.} and Michelle MacLean and MacGregor, {Scott J.} and Anderson, {John G.} and Grant, {M. Helen}",
year = "2016",
month = "11",
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AU - Lamprou, Dimitrios A.

AU - MacLean, Michelle

AU - MacGregor, Scott J.

AU - Anderson, John G.

AU - Grant, M. Helen

PY - 2016/11/30

Y1 - 2016/11/30

N2 - Storage of flexible endoscopes under germicidal ultraviolet (UVC) light has been associated with degradation of device material leading to failure and increased risk to patients. 405 nm germicidal light presents a possible alternative, potentially providing effective bacterial inactivation without material damage. Samples of endoscope material were exposed to UVC and 405 nm germicidal light sources and a broad spectrum light source control. Material properties were monitored using FTIR, AFM, contact angle and confocal microscopy. Significant changes were observed with samples exposed to the UVC source, with variations in FTIR spectra indicative of side chain scission, a decrease in contact angle from 82.6° to 61.4°, an increase in average surface roughness from 2.34 nm to 68.7 nm and visible cracking of the surface. In contrast samples exposed to the 405 nm light source showed little to no changes, with any variations being comparable to those seen on samples exposed to the broad spectrum control. Bacterial adhesion tests on samples showed an 86.8% increase in Pseudomonas aeruginosa adhesion on UVC exposed samples and no significant increase in adhesion with samples exposed to the other light sources. 405 nm germicidal light therefore potentially represents a safer alternative to UVC light for use in flexible endoscope storage.

AB - Storage of flexible endoscopes under germicidal ultraviolet (UVC) light has been associated with degradation of device material leading to failure and increased risk to patients. 405 nm germicidal light presents a possible alternative, potentially providing effective bacterial inactivation without material damage. Samples of endoscope material were exposed to UVC and 405 nm germicidal light sources and a broad spectrum light source control. Material properties were monitored using FTIR, AFM, contact angle and confocal microscopy. Significant changes were observed with samples exposed to the UVC source, with variations in FTIR spectra indicative of side chain scission, a decrease in contact angle from 82.6° to 61.4°, an increase in average surface roughness from 2.34 nm to 68.7 nm and visible cracking of the surface. In contrast samples exposed to the 405 nm light source showed little to no changes, with any variations being comparable to those seen on samples exposed to the broad spectrum control. Bacterial adhesion tests on samples showed an 86.8% increase in Pseudomonas aeruginosa adhesion on UVC exposed samples and no significant increase in adhesion with samples exposed to the other light sources. 405 nm germicidal light therefore potentially represents a safer alternative to UVC light for use in flexible endoscope storage.

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