TY - JOUR
T1 - A comparison of the chromatographic properties of silica gel and silicon hydride modified silica gels
AU - Bawazeer, Saud
AU - Sutcliffe, Oliver B
AU - Euerby, Melvin R
AU - Bawazeer, Sami
AU - Watson, David G
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012/11/9
Y1 - 2012/11/9
N2 - The retention properties of a silica gel column and a type C silica (silicon hydride) column for bases, sugars and polar acids were compared in hydrophilic interaction chromatography (HILIC) mode with formic acid or ammonium acetate as aqueous phase modifiers. The type C silica column was much more retentive for a series of model bases than the silica gel column and, surprisingly, retention of bases increased on the type C silica column when, the higher pH, ammonium acetate containing mobile phase was used. The retention of sugars was greater on the type C silica column than on the silica gel column and also increased on the type C silica column with increased pH suggesting either a silanophilic mechanism of retention or some unknown mechanism. Three type C silica based columns, type C silica, cogent diamond hydride and a β-pinene modified column, which it was hoped might exert some additional stereochemical discrimination, were tested for metabolomic profiling of urine. In general the unmodified type C silica column gave the strongest retention of the many polar metabolites in urine and could provide a useful complement to established HILIC methods for metabolomic profiling.
AB - The retention properties of a silica gel column and a type C silica (silicon hydride) column for bases, sugars and polar acids were compared in hydrophilic interaction chromatography (HILIC) mode with formic acid or ammonium acetate as aqueous phase modifiers. The type C silica column was much more retentive for a series of model bases than the silica gel column and, surprisingly, retention of bases increased on the type C silica column when, the higher pH, ammonium acetate containing mobile phase was used. The retention of sugars was greater on the type C silica column than on the silica gel column and also increased on the type C silica column with increased pH suggesting either a silanophilic mechanism of retention or some unknown mechanism. Three type C silica based columns, type C silica, cogent diamond hydride and a β-pinene modified column, which it was hoped might exert some additional stereochemical discrimination, were tested for metabolomic profiling of urine. In general the unmodified type C silica column gave the strongest retention of the many polar metabolites in urine and could provide a useful complement to established HILIC methods for metabolomic profiling.
KW - silica gel
KW - type C silica
KW - basic compounds
KW - metabolomic profiling
UR - http://www.scopus.com/inward/record.url?scp=84867481745&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2012.09.005
DO - 10.1016/j.chroma.2012.09.005
M3 - Article
C2 - 23040975
VL - 1263
SP - 61
EP - 67
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -