A compact instrument for adjusting laser beams to be accurately coincident and coaxial and its use in biomedical imaging using wave-mixed laser sources

R Amor, G Norris, J Dempster, W B Amos, G McConnell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biomedical imaging applications that involve nonlinear optical processes such as sum-frequency generation (SFG) and four-wave mixing require that the pulses are synchronized in time and the beams are coaxial to better than 400 μrad. For this reason, folding mirrors are normally used to extend the beam path over a few meters so that detectors can be put into the beams to check their overlap at the start of a long path and also at the end of it. We have made a portable instrument with a footprint of only 22 cm × 11 cm × 16 cm that uses a short focal length lens and a telephoto combination for viewing the near-field and far-field simultaneously. Our instrument is simple to build and use, and we show its application in coherent anti-Stokes Raman scattering microscopy and SFG-based two-photon fluorescence microscopy.

LanguageEnglish
Article number083705
Number of pages5
JournalReview of Scientific Instruments
Volume83
Issue number8
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Optical Phenomena
Raman Spectrum Analysis
Photons
Fluorescence Microscopy
Lenses
Laser beams
Microscopy
Lasers
adjusting
laser beams
Imaging techniques
microscopy
lasers
Four wave mixing
Fluorescence microscopy
footprints
four-wave mixing
folding
far fields
Raman scattering

Keywords

  • animals
  • lasers
  • microscopy, fluorescence, multiphoton
  • molecular imaging
  • snails
  • spectrum analysis, raman
  • compact instrument
  • laser beams
  • adjustment

Cite this

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abstract = "Biomedical imaging applications that involve nonlinear optical processes such as sum-frequency generation (SFG) and four-wave mixing require that the pulses are synchronized in time and the beams are coaxial to better than 400 μrad. For this reason, folding mirrors are normally used to extend the beam path over a few meters so that detectors can be put into the beams to check their overlap at the start of a long path and also at the end of it. We have made a portable instrument with a footprint of only 22 cm × 11 cm × 16 cm that uses a short focal length lens and a telephoto combination for viewing the near-field and far-field simultaneously. Our instrument is simple to build and use, and we show its application in coherent anti-Stokes Raman scattering microscopy and SFG-based two-photon fluorescence microscopy.",
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AU - McConnell, G

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