A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart; Emmet J. O'Reilly; Roisin D. Moriarty; Paolo Bertoncello; Tia E. Keyes; Robert J. Forster; Lynn Dennany

doi:10.1016/j.electacta.2015.01.073

A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart, Emmet J. O'Reilly, Roisin D. Moriarty, Paolo Bertoncello, Tia E. Keyes, Robert J. Forster, Lynn Dennany

Pure And Applied Chemistry

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Abstract

This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

Original language	English
Pages (from-to)	8-14
Number of pages	7
Journal	Electrochimica Acta
Volume	157
Early online date	17 Jan 2015
DOIs	https://doi.org/10.1016/j.electacta.2015.01.073
Publication status	Published - 1 Mar 2015

Keywords

electrochemiluminescence (ECL)
biomarkers
quantum dot

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10.1016/j.electacta.2015.01.073

Stewart-etal-EA2015-cholesterol-biosensor-NIR-ECL-quantum-dotsAccepted author manuscript, 1.01 MBLicence: CC BY-NC-ND 4.0

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Stewart, A. J., O'Reilly, E. J., Moriarty, R. D., Bertoncello, P., Keyes, T. E., Forster, R. J., & Dennany, L. (2015). A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. Electrochimica Acta, 157, 8-14. https://doi.org/10.1016/j.electacta.2015.01.073

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title = "A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots",

abstract = "This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future. ",

keywords = "electrochemiluminescence (ECL), biomarkers, quantum dot",

author = "Stewart, {Alasdair J.} and O'Reilly, {Emmet J.} and Moriarty, {Roisin D.} and Paolo Bertoncello and Keyes, {Tia E.} and Forster, {Robert J.} and Lynn Dennany",

note = "NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073",

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AU - Stewart, Alasdair J.

AU - O'Reilly, Emmet J.

AU - Moriarty, Roisin D.

AU - Bertoncello, Paolo

AU - Keyes, Tia E.

AU - Forster, Robert J.

AU - Dennany, Lynn

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073

PY - 2015/3/1

Y1 - 2015/3/1

N2 - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

AB - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

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KW - biomarkers

KW - quantum dot

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