A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart, Emmet J. O'Reilly, Roisin D. Moriarty, Paolo Bertoncello, Tia E. Keyes, Robert J. Forster, Lynn Dennany

Research output: Contribution to journalArticle

32 Citations (Scopus)
183 Downloads (Pure)

Abstract

This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future. 
Original languageEnglish
Pages (from-to)8-14
Number of pages7
JournalElectrochimica Acta
Volume157
Early online date17 Jan 2015
DOIs
Publication statusPublished - 1 Mar 2015

Keywords

  • electrochemiluminescence (ECL)
  • biomarkers
  • quantum dot

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