A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart; Emmet J. O'Reilly; Roisin D. Moriarty; Paolo Bertoncello; Tia E. Keyes; Robert J. Forster; Lynn Dennany

doi:10.1016/j.electacta.2015.01.073

A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart, Emmet J. O'Reilly, Roisin D. Moriarty, Paolo Bertoncello, Tia E. Keyes, Robert J. Forster, Lynn Dennany

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

189 Downloads (Pure)

Abstract

This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

Original language	English
Pages (from-to)	8-14
Number of pages	7
Journal	Electrochimica Acta
Volume	157
Early online date	17 Jan 2015
DOIs	https://doi.org/10.1016/j.electacta.2015.01.073
Publication status	Published - 1 Mar 2015

Keywords

electrochemiluminescence (ECL)
biomarkers
quantum dot

Access to Document

10.1016/j.electacta.2015.01.073

Stewart-etal-EA2015-cholesterol-biosensor-NIR-ECL-quantum-dotsAccepted author manuscript, 1.01 MBLicence: CC BY-NC-ND 4.0

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1 Finished

ECL Nano-Materials
Dennany, L. & Skabara, P.
European Commission - FP7 - Cooperation only
1/10/10 → 30/09/14
Project: Research

2 Organiser of major conference
1 Participation in conference

3rd Annual Translation & Application of Biosciences: Analytical Biosciences ECM
Lynn Dennany (Member of programme committee)
16 Mar 2016 → 17 Mar 2016
Activity: Participating in or organising an event types › Organiser of major conference
Emerging Analytical Professionals RSC
Lynn Dennany (Invited speaker)
8 Apr 2015 → 10 Apr 2015
Activity: Participating in or organising an event types › Participation in conference
2nd Translation & Application of Biosciences: Analytical Biosciences Early Career Researchers Conference 2013
Lynn Dennany (Member of programme committee)
3 Dec 2013 → 4 Dec 2013
Activity: Participating in or organising an event types › Organiser of major conference

Cite this

@article{3312750c531d4df28ebb4b7f7e91f177,

title = "A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots",

abstract = "This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future. ",

keywords = "electrochemiluminescence (ECL), biomarkers, quantum dot",

author = "Stewart, {Alasdair J.} and O'Reilly, {Emmet J.} and Moriarty, {Roisin D.} and Paolo Bertoncello and Keyes, {Tia E.} and Forster, {Robert J.} and Lynn Dennany",

note = " NOTICE: this is the author{\textquoteright}s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073",

year = "2015",

month = mar,

day = "1",

doi = "10.1016/j.electacta.2015.01.073",

language = "English",

volume = "157",

pages = "8--14",

journal = "Electrochimica Acta",

issn = "0013-4686",

}

A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. / Stewart, Alasdair J.; O'Reilly, Emmet J.; Moriarty, Roisin D.; Bertoncello, Paolo; Keyes, Tia E.; Forster, Robert J.; Dennany, Lynn.

In: Electrochimica Acta, Vol. 157, 01.03.2015, p. 8-14.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

AU - Stewart, Alasdair J.

AU - O'Reilly, Emmet J.

AU - Moriarty, Roisin D.

AU - Bertoncello, Paolo

AU - Keyes, Tia E.

AU - Forster, Robert J.

AU - Dennany, Lynn

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073

PY - 2015/3/1

Y1 - 2015/3/1

N2 - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

AB - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.

KW - electrochemiluminescence (ECL)

KW - biomarkers

KW - quantum dot

UR - http://www.sciencedirect.com/science/journal/00134686

U2 - 10.1016/j.electacta.2015.01.073

DO - 10.1016/j.electacta.2015.01.073

M3 - Article

VL - 157

SP - 8

EP - 14

JO - Electrochimica Acta

JF - Electrochimica Acta

SN - 0013-4686

ER -

A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Abstract

Keywords

Access to Document

ECL Nano-Materials

3rd Annual Translation & Application of Biosciences: Analytical Biosciences ECM

Emerging Analytical Professionals RSC

2nd Translation & Application of Biosciences: Analytical Biosciences Early Career Researchers Conference 2013

Cite this