A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots

Alasdair J. Stewart, Emmet J. O'Reilly, Roisin D. Moriarty, Paolo Bertoncello, Tia E. Keyes, Robert J. Forster, Lynn Dennany

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future. 
LanguageEnglish
Pages8-14
Number of pages7
JournalElectrochimica Acta
Volume157
Early online date17 Jan 2015
DOIs
Publication statusPublished - 1 Mar 2015

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Chemiluminescence
Cholesterol
Biosensors
Semiconductor quantum dots
Infrared radiation
Water
Chitosan
Cholesterol Oxidase
Byproducts
Imaging techniques
Oxidation
Sensors

Keywords

  • electrochemiluminescence (ECL)
  • biomarkers
  • quantum dot

Cite this

Stewart, Alasdair J. ; O'Reilly, Emmet J. ; Moriarty, Roisin D. ; Bertoncello, Paolo ; Keyes, Tia E. ; Forster, Robert J. ; Dennany, Lynn. / A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. In: Electrochimica Acta. 2015 ; Vol. 157. pp. 8-14.
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abstract = "This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future. ",
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A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. / Stewart, Alasdair J.; O'Reilly, Emmet J.; Moriarty, Roisin D.; Bertoncello, Paolo; Keyes, Tia E.; Forster, Robert J.; Dennany, Lynn.

In: Electrochimica Acta, Vol. 157, 01.03.2015, p. 8-14.

Research output: Contribution to journalArticle

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AU - Bertoncello, Paolo

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AU - Forster, Robert J.

AU - Dennany, Lynn

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