Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 8-14 |
| Number of pages | 7 |
| Journal | Electrochimica Acta |
| Volume | 157 |
| Early online date | 17 Jan 2015 |
| DOIs | |
| Publication status | Published - 1 Mar 2015 |
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Keywords
- electrochemiluminescence (ECL)
- biomarkers
- quantum dot
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A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots. / Stewart, Alasdair J.; O'Reilly, Emmet J.; Moriarty, Roisin D.; Bertoncello, Paolo; Keyes, Tia E.; Forster, Robert J.; Dennany, Lynn.
In: Electrochimica Acta, Vol. 157, 01.03.2015, p. 8-14.Research output: Contribution to journal › Article
TY - JOUR
T1 - A cholesterol biosensor based on the NIR electrogenerated-chemiluminescence (ECL) of water-soluble CdSeTe/ZnS quantum dots
AU - Stewart, Alasdair J.
AU - O'Reilly, Emmet J.
AU - Moriarty, Roisin D.
AU - Bertoncello, Paolo
AU - Keyes, Tia E.
AU - Forster, Robert J.
AU - Dennany, Lynn
N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Electrochimica Acta. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Electrochimica Acta, 157, (17/01/2015) http://dx.doi.org/10.1016/j.electacta.2015.01.073
PY - 2015/3/1
Y1 - 2015/3/1
N2 - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.
AB - This contribution examines the application of near infra-red (NIR) quantum dot (QD) containing films for cholesterol detection. Water-soluble, 2-(dimethylamino)ethanthiol (DAET) protected 800 nm CdSeTe/ZnS core-shell QDs were prepared and incorporated into a chitosan film. The NIR electrogenerated chemiluminescence (ECL) of the QD/chitosan films upon reaction with H2O2 co-reactant (produced as a by-product of cholesterol oxidase-catalysed oxidation of cholesterol) gave a strong ECL signal at -1.35 V vs. Ag/AgCl. The sensor displayed a linear response over the clinically relevant range (0.25 ≤ [cholesterol] ≤ 5 mM) allowing the rapid detection of cholesterol and providing a platform for future development. Significantly, this NIR emission has been shown to exhibit excellent penetrability through biological samples, and will likely be at the forefront of development in the biosensing and imaging fields for the foreseeable future.
KW - electrochemiluminescence (ECL)
KW - biomarkers
KW - quantum dot
UR - http://www.sciencedirect.com/science/journal/00134686
U2 - 10.1016/j.electacta.2015.01.073
DO - 10.1016/j.electacta.2015.01.073
M3 - Article
VL - 157
SP - 8
EP - 14
JO - Electrochimica Acta
JF - Electrochimica Acta
SN - 0013-4686
ER -