Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes . Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths . Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available . LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.
|Number of pages||1|
|Publication status||Published - 4 Jul 2017|
|Event||Microscience Microscopy Congress 2017 - Manchester, United Kingdom|
Duration: 3 Jul 2017 → 6 Jul 2017
|Conference||Microscience Microscopy Congress 2017|
|Period||3/07/17 → 6/07/17|
- cell imaging
- light emitting diode
- LED wavelength
Tinning, P. W., Franssen, A., Hridi, S. U., Bushell, T., & McConnell, G. (2017). A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. Poster session presented at Microscience Microscopy Congress 2017, Manchester, United Kingdom.