A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

Peter William Tinning; Aimee Franssen; Shehla Unaiza Hridi; Trevor Bushell; Gail McConnell

A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

Peter William Tinning, Aimee Franssen, Shehla Unaiza Hridi, Trevor Bushell, Gail McConnell

Research output: Contribution to conference › Poster › peer-review

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Abstract

Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.

Original language	English
Number of pages	1
Publication status	Published - 4 Jul 2017
Event	Microscience Microscopy Congress 2017 - Manchester, United Kingdom Duration: 3 Jul 2017 → 6 Jul 2017

Conference

Conference	Microscience Microscopy Congress 2017
Country/Territory	United Kingdom
City	Manchester
Period	3/07/17 → 6/07/17

Keywords

cell imaging
light emitting diode
LED wavelength

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Tinning-etal-MMC-2017-A-340-380-nm-light-emitting-diode-illuminator-for-Fura-2-AM-ratiometricFinal published version, 1.42 MB

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title = "A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision",

abstract = "Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.",

keywords = "cell imaging, light emitting diode, LED wavelength",

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A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. / Tinning, Peter William; Franssen, Aimee; Hridi, Shehla Unaiza et al.
2017. Poster session presented at Microscience Microscopy Congress 2017, Manchester, United Kingdom.

Research output: Contribution to conference › Poster › peer-review

TY - CONF

T1 - A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

AU - Tinning, Peter William

AU - Franssen, Aimee

AU - Hridi, Shehla Unaiza

AU - Bushell, Trevor

AU - McConnell, Gail

PY - 2017/7/4

Y1 - 2017/7/4

N2 - Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.

AB - Cytosolic Ca2+ plays an integral role in cells and the study of its dynamics can reveal much about biological processes [1]. Fura-2 can provide quantitative data on cytosolic Ca2+ changes by exciting at 340 nm and 380 nm and taking the ratio of the emission at both wavelengths [2]. Traditionally for this type of imaging an arc lamp had to be used for illumination as LEDs of the appropriate wavelengths were not available [3]. LEDs hold advantages over arc lamps by exhibiting high amplitude stability and the ability to rapidly switch between wavelengths. We aimed to test a new 340/380 nm LED system for use in ratiometric Fura-2 AM Ca2+ imaging and present results using tsA-201 cells and hippocampal neurons.

KW - cell imaging

KW - light emitting diode

KW - LED wavelength

UR - http://www.mmc-series.org.uk/

M3 - Poster

T2 - Microscience Microscopy Congress 2017

Y2 - 3 July 2017 through 6 July 2017

ER -

A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

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Keywords

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