8-hydroxyquinolinyl azo dyes: a class of surface-enhanced resonance raman scattering-based probes for ultrasensitive monitoring of enzymatic activity

A.M. Ingram, Robert J. Stokes, J. Redden, K. Gibson, B.D. Moore, K. Faulds, D. Graham

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A series of surface-enhanced resonance Raman scattering (SERRS) based probes for the detection of lipase activity are reported. A number of novel SERRS-active 8-hydroxylquinolinyl azo dyes have been prepared and via synthetic esterification or subsequent enzymatic hydrolysis at the 8-hydroxyl position the SERRS signal can be 'switched' on or off. In the first instance, the technique has been demonstrated for the successful detection of lipase from Pseudomonas cepacia, and these new compounds offer a limit of detection of 0.2 ng mL-1 enzyme, up to a 100-fold lower limit than observed for benzotriazolyl dyes used in previous studies. The chemical synthesis is straightforward and allows for facile introduction of a wide range of different masking groups, using commonly known synthetic methodologies. The potential for multiplexing analysis of enzyme activity using this technology is presented within.
LanguageEnglish
Pages8578-8583
Number of pages5
JournalAnalytical Chemistry
Volume79
Issue number22
DOIs
Publication statusPublished - 15 Nov 2007

Fingerprint

Azo Compounds
Raman scattering
Lipase
Monitoring
Enzymatic hydrolysis
Esterification
Enzyme activity
Multiplexing
Hydroxyl Radical
Coloring Agents
Enzymes

Keywords

  • surface-enhanced resonance Raman scattering
  • SERRS
  • benzotriazolyl dye
  • multiplexing analysis
  • enzyme activity
  • 8-hydroxyquinolinyl azo dyes

Cite this

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title = "8-hydroxyquinolinyl azo dyes: a class of surface-enhanced resonance raman scattering-based probes for ultrasensitive monitoring of enzymatic activity",
abstract = "A series of surface-enhanced resonance Raman scattering (SERRS) based probes for the detection of lipase activity are reported. A number of novel SERRS-active 8-hydroxylquinolinyl azo dyes have been prepared and via synthetic esterification or subsequent enzymatic hydrolysis at the 8-hydroxyl position the SERRS signal can be 'switched' on or off. In the first instance, the technique has been demonstrated for the successful detection of lipase from Pseudomonas cepacia, and these new compounds offer a limit of detection of 0.2 ng mL-1 enzyme, up to a 100-fold lower limit than observed for benzotriazolyl dyes used in previous studies. The chemical synthesis is straightforward and allows for facile introduction of a wide range of different masking groups, using commonly known synthetic methodologies. The potential for multiplexing analysis of enzyme activity using this technology is presented within.",
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8-hydroxyquinolinyl azo dyes: a class of surface-enhanced resonance raman scattering-based probes for ultrasensitive monitoring of enzymatic activity. / Ingram, A.M.; Stokes, Robert J.; Redden, J.; Gibson, K.; Moore, B.D.; Faulds, K.; Graham, D.

In: Analytical Chemistry, Vol. 79, No. 22, 15.11.2007, p. 8578-8583.

Research output: Contribution to journalArticle

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T1 - 8-hydroxyquinolinyl azo dyes: a class of surface-enhanced resonance raman scattering-based probes for ultrasensitive monitoring of enzymatic activity

AU - Ingram, A.M.

AU - Stokes, Robert J.

AU - Redden, J.

AU - Gibson, K.

AU - Moore, B.D.

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AU - Graham, D.

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AB - A series of surface-enhanced resonance Raman scattering (SERRS) based probes for the detection of lipase activity are reported. A number of novel SERRS-active 8-hydroxylquinolinyl azo dyes have been prepared and via synthetic esterification or subsequent enzymatic hydrolysis at the 8-hydroxyl position the SERRS signal can be 'switched' on or off. In the first instance, the technique has been demonstrated for the successful detection of lipase from Pseudomonas cepacia, and these new compounds offer a limit of detection of 0.2 ng mL-1 enzyme, up to a 100-fold lower limit than observed for benzotriazolyl dyes used in previous studies. The chemical synthesis is straightforward and allows for facile introduction of a wide range of different masking groups, using commonly known synthetic methodologies. The potential for multiplexing analysis of enzyme activity using this technology is presented within.

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KW - benzotriazolyl dye

KW - multiplexing analysis

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