5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin

N.V. Visser, A.H. Westphal, S.M. Nabuurs, A. van Hoek, C.P.M. van Mierlo, A.J.W.G. Visser, J. Broos, H. van Amerongen

Research output: Contribution to journalArticle

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Abstract

The apoflavodoxin protein from Azotobacter vinelandii harboring three tryptophan (Trp) residues, was biosynthetically labeled with 5-fluorotryptophan (5-FTrp). 5-FTrp has the advantage that chemical differences in its microenvironment can be sensitively visualized via F-19 NMR. Moreover, it shows simpler fluorescence decay kinetics. The occurrence of FRET was earlier observed via the fluorescence anisotropy decay of WT apoflavodoxin and the anisotropy decay parameters are in excellent agreement with distances between and relative orientations of all Trp residues. The anisotropy decay in 5-FTrp apoflavodoxin demonstrates that the distances and orientations are identical for this protein. This work demonstrates the added value of replacing Trp by 5-FTrp to study structural features of proteins via F-19 NMR and fluorescence spectroscopy.
LanguageEnglish
Pages2785-2788
Number of pages3
JournalFEBS Letters
Volume583
Issue number17
DOIs
Publication statusPublished - 3 Sep 2009

Fingerprint

Excited states
Ground state
Tryptophan
Anisotropy
Fluorescence
Azotobacter vinelandii
Proteins
Fluorescence Polarization
Fluorescence Spectrometry
Fluorescence spectroscopy
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Kinetics
apoflavodoxin
5-fluorotryptophan

Keywords

  • fluorescence anisotropy
  • F-19 NMR
  • picosecond fluorescence
  • protein folding
  • protein stability
  • azotobacter-vinelandii
  • fluorescence decay
  • tryptophan
  • proteins
  • kinetics
  • pathway

Cite this

Visser, N. V., Westphal, A. H., Nabuurs, S. M., van Hoek, A., van Mierlo, C. P. M., Visser, A. J. W. G., ... van Amerongen, H. (2009). 5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin. FEBS Letters, 583(17), 2785-2788. https://doi.org/10.1016/j.febslet.2009.07.022
Visser, N.V. ; Westphal, A.H. ; Nabuurs, S.M. ; van Hoek, A. ; van Mierlo, C.P.M. ; Visser, A.J.W.G. ; Broos, J. ; van Amerongen, H. / 5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin. In: FEBS Letters. 2009 ; Vol. 583, No. 17. pp. 2785-2788.
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abstract = "The apoflavodoxin protein from Azotobacter vinelandii harboring three tryptophan (Trp) residues, was biosynthetically labeled with 5-fluorotryptophan (5-FTrp). 5-FTrp has the advantage that chemical differences in its microenvironment can be sensitively visualized via F-19 NMR. Moreover, it shows simpler fluorescence decay kinetics. The occurrence of FRET was earlier observed via the fluorescence anisotropy decay of WT apoflavodoxin and the anisotropy decay parameters are in excellent agreement with distances between and relative orientations of all Trp residues. The anisotropy decay in 5-FTrp apoflavodoxin demonstrates that the distances and orientations are identical for this protein. This work demonstrates the added value of replacing Trp by 5-FTrp to study structural features of proteins via F-19 NMR and fluorescence spectroscopy.",
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Visser, NV, Westphal, AH, Nabuurs, SM, van Hoek, A, van Mierlo, CPM, Visser, AJWG, Broos, J & van Amerongen, H 2009, '5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin' FEBS Letters, vol. 583, no. 17, pp. 2785-2788. https://doi.org/10.1016/j.febslet.2009.07.022

5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin. / Visser, N.V.; Westphal, A.H.; Nabuurs, S.M.; van Hoek, A.; van Mierlo, C.P.M.; Visser, A.J.W.G.; Broos, J.; van Amerongen, H.

In: FEBS Letters, Vol. 583, No. 17, 03.09.2009, p. 2785-2788.

Research output: Contribution to journalArticle

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T1 - 5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin

AU - Visser, N.V.

AU - Westphal, A.H.

AU - Nabuurs, S.M.

AU - van Hoek, A.

AU - van Mierlo, C.P.M.

AU - Visser, A.J.W.G.

AU - Broos, J.

AU - van Amerongen, H.

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Visser NV, Westphal AH, Nabuurs SM, van Hoek A, van Mierlo CPM, Visser AJWG et al. 5-fluorotryptophan as dual probe for ground-state heterogeneity and excited-state dynamics in apoflavodoxin. FEBS Letters. 2009 Sep 3;583(17):2785-2788. https://doi.org/10.1016/j.febslet.2009.07.022