4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Giuseppe Di Caprio; Ahmed El Mallahi; Pietro Ferraro; Roberta Dale; Gianfranco Coppola; Brian Dale; Giuseppe Coppola; Frank Dubois

doi:10.1364/BOE.5.000690

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Giuseppe Di Caprio, Ahmed El Mallahi, Pietro Ferraro, Roberta Dale, Gianfranco Coppola, Brian Dale, Giuseppe Coppola, Frank Dubois

Biomedical Engineering

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Abstract

In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

Original language	English
Pages (from-to)	690-700
Number of pages	11
Journal	Biomedical Optics Express
Volume	5
Issue number	3
Early online date	11 Feb 2014
DOIs	https://doi.org/10.1364/BOE.5.000690
Publication status	Published - 31 Mar 2014

Keywords

sperm analysis
digital holography
cell motility

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10.1364/BOE.5.000690

Di-Caprio-etal-BOE-2014-4D-tracking-of-clinical-seminal-samples-for-quantitativeFinal published version, 3.07 MBLicence: CC BY 4.0

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title = "4D tracking of clinical seminal samples for quantitative characterization of motility parameters",

abstract = "In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.",

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AU - Di Caprio, Giuseppe

AU - El Mallahi, Ahmed

AU - Ferraro, Pietro

AU - Dale, Roberta

AU - Coppola, Gianfranco

AU - Dale, Brian

AU - Coppola, Giuseppe

AU - Dubois, Frank

PY - 2014/3/31

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N2 - In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

AB - In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.

KW - sperm analysis

KW - digital holography

KW - cell motility

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DO - 10.1364/BOE.5.000690

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 3

ER -

4D tracking of clinical seminal samples for quantitative characterization of motility parameters

Abstract

Keywords

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