454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes

Fabrizio Colosimo, Richard Lord, Russell Thomas, Jonathan R. Lloyd, Christopher Boothman, Robert M Kalin

Research output: Contribution to journal › Article

Abstract

Anaerobic treatment process is a cost-effective method for treating organic wastes, since the biogas formed can be used for heat/electricity production and the digester residues can be recycled for other applications. An innovative use of the digestate could be as biodegradative and methanogenic inoculum for the stimulation of methane production in gas-producing or depleted wells. The microbial communities involved in the biodegradation of petrochemical waste are similar to the indigenous microorganisms typically found in unconventional basins. These communities also follow the same cascade of reactions: from the initial breakdown of complex molecules to the production of intermediate compounds used by methanogens. This study carried out a culture-independent assessment of the bacterial community composition of a digestate from the Bran Sands Advanced Digestion Facility (Middleborough, UK) and compared the results with the microbial populations found in unconventional gas basins. The 454 pyrosequencing analyses revealed a bacterial community dominated by Thermotogae, Bacteroidia, Clostridia and Synergistia, which are typically found in unconventional gas systems. The classification of nucleotide sequence reads and assembled contigs revealed a genetic profile characteristic for an anaerobic microbial consortium running fermentative metabolic pathways. The assignment of numerous sequences was related to hydrocarbon decomposition and digestion of cellulosic material, which indicates that the bacterial community is engaged in hydrolysis of plant-derived material. The bacterial community composition suggest that the effluent of the digester can be used as a biodegradative inoculum for the stimulation of methane generation in unconventional wells, where events of microbial methanogenesis have been previously observed.
LanguageEnglish
JournalEnergy and Environment Research
Publication statusAccepted/In press - 6 Mar 2017

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Hydrolysis
Bacteria
Hot Temperature
Gases
Methane
Digestion
Microbial Consortia
Methanogens
Clostridium
Electricity
Biofuels
Thermogenesis
Biogas
Nucleotides
Hydrocarbons
Metabolic Networks and Pathways
Biodegradation
Chemical analysis
Petrochemicals
Microorganisms

Keywords

  • pyrosequencing
  • anaerobic degradation
  • petrochemical waste
  • microbial populations
  • hydrocarbon decomposition
  • cellulosic material
  • microbial methanogenesis

Cite this

Colosimo, Fabrizio ; Lord, Richard ; Thomas, Russell ; Lloyd, Jonathan R. ; Boothman, Christopher ; Kalin, Robert M. / 454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes. In: Energy and Environment Research. 2017.
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Colosimo, F, Lord, R, Thomas, R, Lloyd, JR, Boothman, C & Kalin, RM 2017, '454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes' Energy and Environment Research.

454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes. / Colosimo, Fabrizio; Lord, Richard; Thomas, Russell; Lloyd, Jonathan R.; Boothman, Christopher; Kalin, Robert M.

In: Energy and Environment Research, 06.03.2017.

Research output: Contribution to journal › Article

TY - JOUR

T1 - 454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes

AU - Colosimo, Fabrizio

AU - Lord, Richard

AU - Thomas, Russell

AU - Lloyd, Jonathan R.

AU - Boothman, Christopher

AU - Kalin, Robert M

PY - 2017/3/6

Y1 - 2017/3/6

N2 - Anaerobic treatment process is a cost-effective method for treating organic wastes, since the biogas formed can be used for heat/electricity production and the digester residues can be recycled for other applications. An innovative use of the digestate could be as biodegradative and methanogenic inoculum for the stimulation of methane production in gas-producing or depleted wells. The microbial communities involved in the biodegradation of petrochemical waste are similar to the indigenous microorganisms typically found in unconventional basins. These communities also follow the same cascade of reactions: from the initial breakdown of complex molecules to the production of intermediate compounds used by methanogens. This study carried out a culture-independent assessment of the bacterial community composition of a digestate from the Bran Sands Advanced Digestion Facility (Middleborough, UK) and compared the results with the microbial populations found in unconventional gas basins. The 454 pyrosequencing analyses revealed a bacterial community dominated by Thermotogae, Bacteroidia, Clostridia and Synergistia, which are typically found in unconventional gas systems. The classification of nucleotide sequence reads and assembled contigs revealed a genetic profile characteristic for an anaerobic microbial consortium running fermentative metabolic pathways. The assignment of numerous sequences was related to hydrocarbon decomposition and digestion of cellulosic material, which indicates that the bacterial community is engaged in hydrolysis of plant-derived material. The bacterial community composition suggest that the effluent of the digester can be used as a biodegradative inoculum for the stimulation of methane generation in unconventional wells, where events of microbial methanogenesis have been previously observed.

AB - Anaerobic treatment process is a cost-effective method for treating organic wastes, since the biogas formed can be used for heat/electricity production and the digester residues can be recycled for other applications. An innovative use of the digestate could be as biodegradative and methanogenic inoculum for the stimulation of methane production in gas-producing or depleted wells. The microbial communities involved in the biodegradation of petrochemical waste are similar to the indigenous microorganisms typically found in unconventional basins. These communities also follow the same cascade of reactions: from the initial breakdown of complex molecules to the production of intermediate compounds used by methanogens. This study carried out a culture-independent assessment of the bacterial community composition of a digestate from the Bran Sands Advanced Digestion Facility (Middleborough, UK) and compared the results with the microbial populations found in unconventional gas basins. The 454 pyrosequencing analyses revealed a bacterial community dominated by Thermotogae, Bacteroidia, Clostridia and Synergistia, which are typically found in unconventional gas systems. The classification of nucleotide sequence reads and assembled contigs revealed a genetic profile characteristic for an anaerobic microbial consortium running fermentative metabolic pathways. The assignment of numerous sequences was related to hydrocarbon decomposition and digestion of cellulosic material, which indicates that the bacterial community is engaged in hydrolysis of plant-derived material. The bacterial community composition suggest that the effluent of the digester can be used as a biodegradative inoculum for the stimulation of methane generation in unconventional wells, where events of microbial methanogenesis have been previously observed.

KW - pyrosequencing

KW - anaerobic degradation

KW - petrochemical waste

KW - microbial populations

KW - hydrocarbon decomposition

KW - cellulosic material

KW - microbial methanogenesis

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M3 - Article

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SN - 1927-0569

ER -

Colosimo F, Lord R, Thomas R, Lloyd JR, Boothman C, Kalin RM. 454 pyrosequencing assessment of biodegradative bacteria from thermal hydrolysis processes. Energy and Environment Research. 2017 Mar 6.

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