@inproceedings{2b9f139ecd9e4765a91b85897e7d8053,
title = "3D visualization and biovolume estimation of motile cells by digital holography",
abstract = "For the monitoring of biological samples, physical parameters such as size, shape and refractive index are of crucial importance. However, up to now the morphological in-vitro analysis of in-vitro cells has been limited to 2D analysis by classical optical microscopy such as phase-contrast or DIC. Here we show an approach that exploits the capability of optical tweezers to trap and put in self-rotation bovine spermatozoa flowing into a microfluidic channel. At same time, digital holographic microscopy allows to image the cell in phase-contrast modality for each different angular position, during the rotation. From the collected information about the cell{\^a}{\texttrademark}s phase-contrast signature, we demonstrate that it is possible to reconstruct the 3D shape of the cell and estimate its volume. The method can open new pathways for rapid measurement of in-vitro cells volume in microfluidic lab-on-a-chip platform, thus having access to 3D shape of the object avoiding tomography microscopy, that is an overwhelmed and very complex approach for measuring 3D shape and biovolume estimation.",
keywords = "biovolume, digital holography, microfluidics, optical tweezers",
author = "F. Merola and L. Miccio and P. Memmolo and {Di Caprio}, G. and G. Coppola and P. Netti",
year = "2014",
month = may,
day = "8",
doi = "10.1117/12.2052836",
language = "English",
isbn = "9781628410778",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
editor = "J{\"u}rgen Popp and Tuchin, {Valery V.} and Matthews, {Dennis L.} and Pavone, {Francesco Saverio} and Paul Garside",
booktitle = "Biophotonics",
note = "Biophotonics: Photonic Solutions for Better Health Care IV ; Conference date: 14-04-2014 Through 17-04-2014",
}