Abstract
Recent studies have clearly demonstrated that Raman and surface enhanced Raman scattering (SERS) spectroscopies are information rich, non-destructive techniques for the monitoring of subtle intracellular changes. However, despite the demonstrated and sophisticated applications of these techniques in cell studies there still remains a lack of accompanying 3D images. Herein we demonstrate for the first time combined 3D Raman and SERS imaging for the simultaneous confirmation of the cellular inclusion and multiple component detection of SERS nanotags. We also report on the 3D elucidation of the cell nuclei by multivariate analysis methods. Imaging in 3D will be critical to understanding architectural changes between diseased and healthy cells, and tissues. It will also provide non-destructive definitive proof of cellular uptake whilst simultaneously confirming targeting of SERS nanotags to their intended destinations.
| Language | English |
|---|---|
| Pages | 3566-3572 |
| Number of pages | 7 |
| Journal | Chemical Science |
| Volume | 4 |
| Issue number | 9 |
| Early online date | 10 Jul 2013 |
| DOIs | |
| Publication status | Published - 1 Sep 2013 |
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Keywords
- surface enhanced Raman scattering
- SERS
- 3D optical imaging
- cell imaging
Cite this
}
3D optical imaging of multiple SERS nanotags in cells. / McAughtrie, Sarah; Lau, Katherine; Faulds, Karen; Graham, Duncan.
In: Chemical Science, Vol. 4, No. 9, 01.09.2013, p. 3566-3572.Research output: Contribution to journal › Article
TY - JOUR
T1 - 3D optical imaging of multiple SERS nanotags in cells
AU - McAughtrie, Sarah
AU - Lau, Katherine
AU - Faulds, Karen
AU - Graham, Duncan
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Recent studies have clearly demonstrated that Raman and surface enhanced Raman scattering (SERS) spectroscopies are information rich, non-destructive techniques for the monitoring of subtle intracellular changes. However, despite the demonstrated and sophisticated applications of these techniques in cell studies there still remains a lack of accompanying 3D images. Herein we demonstrate for the first time combined 3D Raman and SERS imaging for the simultaneous confirmation of the cellular inclusion and multiple component detection of SERS nanotags. We also report on the 3D elucidation of the cell nuclei by multivariate analysis methods. Imaging in 3D will be critical to understanding architectural changes between diseased and healthy cells, and tissues. It will also provide non-destructive definitive proof of cellular uptake whilst simultaneously confirming targeting of SERS nanotags to their intended destinations.
AB - Recent studies have clearly demonstrated that Raman and surface enhanced Raman scattering (SERS) spectroscopies are information rich, non-destructive techniques for the monitoring of subtle intracellular changes. However, despite the demonstrated and sophisticated applications of these techniques in cell studies there still remains a lack of accompanying 3D images. Herein we demonstrate for the first time combined 3D Raman and SERS imaging for the simultaneous confirmation of the cellular inclusion and multiple component detection of SERS nanotags. We also report on the 3D elucidation of the cell nuclei by multivariate analysis methods. Imaging in 3D will be critical to understanding architectural changes between diseased and healthy cells, and tissues. It will also provide non-destructive definitive proof of cellular uptake whilst simultaneously confirming targeting of SERS nanotags to their intended destinations.
KW - surface enhanced Raman scattering
KW - SERS
KW - 3D optical imaging
KW - cell imaging
UR - http://www.scopus.com/inward/record.url?scp=84886840611&partnerID=8YFLogxK
U2 - 10.1039/c3sc51437d
DO - 10.1039/c3sc51437d
M3 - Article
VL - 4
SP - 3566
EP - 3572
JO - Chemical Science
T2 - Chemical Science
JF - Chemical Science
SN - 2041-6520
IS - 9
ER -