3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, G. Coppola, P. Netti, P. Ferraro

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

2 Citations (Scopus)

Abstract

We present the possibility to trap cells (mouse fibroblasts, bovine spermatozoa and diatoms), to manage their position and to induce rotation, by using optical tweezers. The aim is to place them in desired positions, in order to record holographic images in a microscope configuration. Then we are able to recover the 3D shape and to calculate the biovolume of the cells starting from the reconstructed quantitative phase maps (QPMs).

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
Place of PublicationBellingham, WA
Number of pages8
DOIs
Publication statusPublished - 4 Mar 2014
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII - San Francisco, CA, United States
Duration: 3 Feb 20146 Feb 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8947
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Country/TerritoryUnited States
CitySan Francisco, CA
Period3/02/146/02/14

Keywords

  • biovolume
  • digital Holography
  • microfluidics
  • optical tweezers

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