Abstract
Biofabrication has been receiving a great deal of attention in tissue engineering and regenerative medicine either by manual or automated processes. Different automated biofabrication techniques have been used to produce cell-laden alginate hydrogel structures, especially bioprinting approaches. These approaches have been limited to 2D or simple 3D structures, however. In this chapter, a novel bioprinting technique is disclosed for the production of more complex alginate hydrogel structures. This was achieved by dividing the alginate hydrogel cross-linking process into three stages: primary calcium ion cross-linking for printability of the gel, secondary calcium ion cross-linking for rigidity of the alginate hydrogel immediately after printing, and tertiary barium ion cross-linking for the long-term stability of the alginate hydrogel in the culture medium.
Original language | English |
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Title of host publication | Computer-Aided Tissue Engineering |
Editors | Alberto Rainer, Lorenzo Moroni |
Place of Publication | New York, NY |
Publisher | Springer |
Pages | 143-148 |
Number of pages | 6 |
ISBN (Electronic) | 9781071606117 |
ISBN (Print) | 9781071606100 |
DOIs | |
Publication status | Published - 2021 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2147 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Funding
AGT acknowledges the scholarship support by Heriot-Watt University. DJC acknowledges the scholarship from Medical Research Scotland.
Keywords
- 3D bioprinting
- alginate hydrogel
- bioextrusion
- biofabrication