3D bioprinting of complex, cell-laden alginate constructs

Atabak Ghanizadeh Tabriz, Dirk Jan Cornelissen, Wenmiao Shu

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Biofabrication has been receiving a great deal of attention in tissue engineering and regenerative medicine either by manual or automated processes. Different automated biofabrication techniques have been used to produce cell-laden alginate hydrogel structures, especially bioprinting approaches. These approaches have been limited to 2D or simple 3D structures, however. In this chapter, a novel bioprinting technique is disclosed for the production of more complex alginate hydrogel structures. This was achieved by dividing the alginate hydrogel cross-linking process into three stages: primary calcium ion cross-linking for printability of the gel, secondary calcium ion cross-linking for rigidity of the alginate hydrogel immediately after printing, and tertiary barium ion cross-linking for the long-term stability of the alginate hydrogel in the culture medium.

Original languageEnglish
Title of host publicationComputer-Aided Tissue Engineering
EditorsAlberto Rainer, Lorenzo Moroni
Place of PublicationNew York, NY
Number of pages6
ISBN (Electronic)9781071606117
ISBN (Print)9781071606100
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • 3D bioprinting
  • alginate hydrogel
  • bioextrusion
  • biofabrication


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