2D-IR spectroscopy: ultrafast insights into biomolecule structure and function

Neil T. Hunt

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Ultrafast 2D-IR spectroscopy is rapidly becoming a valuable tool for examining the relationship between structure and function of biomolecules. The unique combination of molecular-level structural information and ultrafast time resolution gives previously inaccessible insights relating to the rapid structural fluctuations, vibrational dynamics and solvent-solute interactions of proteins, all of which have important implications for the biological function of these species. In this tutorial review, the method and development of ultrafast 2D-IR spectroscopy is discussed, including an introduction to the two main experimental approaches, double resonance and photon echo 2D-IR, and the extension of the technique to non-equilibrium or transient 2D-IR measurements. The scope of the new information available through 2D-IR spectroscopy is then demonstrated by reference to the current state of the art of 2D-IR studies of molecules of biological interest.
LanguageEnglish
Pages1837-1848
Number of pages11
JournalChemical Society Reviews
Volume38
Issue number7
DOIs
Publication statusPublished - 2009

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Biomolecules
Infrared spectroscopy
Photons
Molecules
Proteins

Keywords

  • 2-dimensional infrared-spectroscopy
  • 2d ir spectroscopy
  • vibrational echo spectroscopy
  • islet amyloid polypeptide
  • hydrogen-bond
  • secondary structure
  • alpha-helix
  • dynamics
  • peptides
  • isotopomers

Cite this

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2D-IR spectroscopy: ultrafast insights into biomolecule structure and function. / Hunt, Neil T.

In: Chemical Society Reviews, Vol. 38, No. 7, 2009, p. 1837-1848.

Research output: Contribution to journalArticle

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